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Abstract. Thermodynamic, hydrodynamic and rheological interactions between velocity-dependent thermostats of Lowe-Andersen ( LA ) and Nosé-Hoover-Lowe-Andersen (NHLA), and modified Lees-Edwards (M-LEC ...
the WT. Additionally, mutation V193R introduced into the PM made the mutant more thermostable . Unfortunately, mutation M155V did not have a similar effect on thermostability as V193R when introduced ...
protein was produced in E. coli. The recombinant protein exhibited thermostable PMM and PGM activities, and was able to function with a variety of different metal ions. Comparison between the native ...
described previously was performed. The residual activities were calculated by comparison with the activity in 50 mM Tris–HCl buffer, pH 8.0, without preincubation. Effect of organic solvents on lipase ...
. As shown in Table 3, comparison of the Km and kcat values of the GlcNAc-1-P UTase activity of the ST0452 protein with that of the bacterial GlmU enzyme showed that the thermostable ST0452 protein was very ...
domain (DD). CelK without the DD, (CBDIV–Gh9)CelK and CBDIVCelK bound cellulose. The thermostability of (CBDIV–Gh9)CelK was significantly higher than that of CBDIVCelK and Gh9CelK. The temperature optima ...
) protoplasts. The effect of mercury ions on protoplasts and vacuoles is also discussed. MATERIALS AND METHODS Young seedlings from rape (Brassica napus), flax (Linum usitatissimum), and wheat (Triticum aestivum ...
Abstract A gene encoding the thermostable raw starch digesting α-amylase in Thermobifida fusca NTU22 was amplified by PCR, sequenced and cloned into Pichia pastoris X-33 host strain using the vector ...
and Elongator dependent U34 modifications confer growth inhibition by the tRNase activity of zymocin whereas defects in Kti12 and Elongator cause resistance to the toxin complex (66). Analysis of the effect ...
in conformation due to heat treatment. To further investigate the thermostability of the produced xylanase, and in order to rationalise the effect of heat pre-treatment, the thermal unfolding of the polypeptide ...
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