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of the human prion protein in a dimeric form was reported. Here we report for the first time the overexpression of a human PrP dimer covalently linked by a FLAG peptide (PrP::FLAG::PrP) in the methylotrophic ...
characterization and extended work of sequence analysis. Figure 3. View largeDownload slide An early assay method for viroid. A crude RNA extract from a PSTVd-infected tomato plant was separated by denaturing PAGE ...
chromatography can enable removal of denaturant and folding at the same time. Where the native protein contains disulphide bonds, their appropriate formation is essential. The E. coli cytoplasm is characterized ...
). The molten globule state is an intermediate stage where a protein is “partly unfolded,” and it is thought that proteins are in the molten globule state when recognized by chaperones or for membrane ...
, approved and marketed for human therapies, are produced in bacterial cells (Ferrer-Miralles et al.2009; Overton 2014; Sanchez-Garcia et al. 2016). Many vaccines are based on conventional recombinant proteins ...
resolved by BN-PAGE followed by 35S-phosphorimaging to display their oligomeric state (D). Blue ovals schematically illustrate the oligomeric states of the non- denatured and partially denatured α1 GlyR. (E ...
-homologous end joining (NHEJ). We characterized LINP1 structure and flexibility and analyzed interactions with the NHEJ factor Ku70/Ku80 (Ku) and Ku complexes that direct NHEJ. LINP1 self-assembles into phase ...
, the intrinsically disordered domain of prions prevents abnormal protein aggregation (irreversible denaturation ) by forming droplets (86) mitigated by RNA (87). The primary component of droplets has been thought ...
for the γ-secretase and can severely trigger the Aβ1–42 formation and aggravate pro-amyloidogenicity [8]. The common Aβ theory of AD in humans is complemented by the intraneuronal formation ...
alkaline proteases from other Bacillus sp. have also been extensively studied, characterized , and commercialized [224]. Commercially, proteases find major uses as components of detergent formulations ...
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