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fragments , and the digestion of N - terminal cleavage fragment by TEV PR. Resulted short fragments can be subjected to MALDI TOF-MS analysis. The recombinant substrate is identical with that one shown in figure ...
of the ssDNA. A C- terminal sub- fragment of EndoTT (residues 107–216) had both endonucleolytic and DNA-binding activity, whereas an N - terminal sub- fragment (residues 1–110) displayed only ssDNA-binding activity ...
at this time how the N - terminal GST or 6His/FLAG may affect the kinetic properties of the enzyme, though we have observed that the substrate specificity of ZmIPK1 was not affected by the GST tag. Apart from ...
on the subsite preferences of the enzyme. BSA was cleaved into several fragments . Four of them were N - terminally sequenced, and scissile bonds were determined (Table 1). None of the four cleavage sites contained ...
characterization of a N - terminal fragment ( p5 ) cleaved from fibroblast growth factor-binding protein (FGF-BP) in bovine milk in vitro, Biochim. Biophys. Acta , 2007, vol. 1770 (pg. 1219- 1229) Google Scholar ...
structural studies of N - terminally truncated TERTs from Candida albicans and Candida tropicalis in apo form and complexed with their respective TWJs in several conformations. We found that Candida TERT ...
DNA contain clues that can reflect their molecular history and the fragmentation processes that created them (1–5). To characterize DNA termini via high-throughput sequencing, DNA molecules must first ...
industries [2, 3]. Several bacterial lipases have been purified and biochemically characterized , and their corresponding genes have been cloned and sequenced [4–7]. The application of alkaline lipases ...
Carlsberg. The first 15 amino acid residues of N - terminal sequence of the enzyme were A-Q-S-V-P-Y-G-I-P-Q-I-K-A-P-A, which are different from that of other known fibrinolytic enzymes. The amidolytic ...
(Supplementary Table SI). In-frame cDNA clone of MTS- P5 was obtained from resultant two PCR fragments using their restriction sites and subcloned into the mammalian cell expression vector pcDNA3 (Invitrogen). Cell ...
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