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the cell at rate kre. Viral RNA translation and replication (Eqs 3 to 13) are modeled based on our published HCV and DENV models [19,55]. In brief, our model describes the translation-associated processes ...
significant HCV viral titers on day 5 and day 9 but not on day 14. So, cDNA of four different cell lines P2X4/ HCV , P2X4/NR, NV/ HCV and NV/NR after day 5 of inoculation and day 9 of inoculation were processed ...
with the indicated in vitro transcribed HCV RNA of the indicated recombinants. Viral spread was monitored by immunostainings of infected cells and sequence analysis was performed on amplified envelope protein encoding ...
annealing regulates the HCV lifecycle. Small RNAs that annealed to different locations on the 5′ UTR promoted virus replication with different efficiencies, and we found that replication promotion correlated ...
[32], [33]. Thus, we next investigated whether recombination in vitro could also occur between isolates of different genotypes. Since efficient replication in the infectious cell culture system ...
Fig 6. CAPN5 and CBLB support a postbinding step during HCV lipoviroparticle entry. (A) Schematic overview of the experimental setup used to analyze different steps of the HCV life cycle in the CRISPR ...
of ATG14L knockdown on HCV replication . (A) Huh7-Lunet/T7 cells were treated with 3 different siRNAs against ATG14L twice, and a subgenomic replicon RNA containing firefly luciferase gene (sgJFH1-Fluc, [8, 38 ...
.-silenced cells . (C) Effect of HNRNPK silencing on HCV RNA replication or (D) virus particle production. Huh7-Lunet cells were co-electroporated with 2.5 µM of indicated siRNA and JcR2a in vitro transcripts ...
reporter replicon (sgJFH1-Fluc) (Fig 1B). In vitro transcripts of this replicon were transfected into Huh7-Lunet cells and RNA replication was monitored by determining luciferase activity 4, 12, 18, 24, 48 ...
. As a model of RNA replication , the best-characterized member of this family is hepatitis C virus ( HCV ), a human pathogen that causes chronic liver disease, cirrhosis, and liver cancer, and is a major target ...
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