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Introduction In this communication, we describe improved light detection using a very sensitive electronic device (photon counting camera system Argus 100), which detects 105 times lower concentrations of protein than conventional methods. Materials and Methods Synthetic ZMuciferin (Photinus pyralis) and D-luciferin-O-phosphate were from Novabiochem AG, CH-4448 Läufelfingen, Switzerland. ATP, alkaline phosphatase1) (calf intestine; 200400 U/mg; 25 °C). Anti-rabbit IgG goat immunoglobulin alkaline phosphatase conjugate and rabbit immunoglobulin G were from Sigma, Taufkirchen, FRG. Luciferase1) (Photinus pyralis', spec. act. 8 mU/mg) was a product of Boehringer, Mannheim, FRG. Nitrocellulose filters (BA 85, 0.45 Schleicher and Schul l, Dassel. ) were products of Protein blot analyses are now extensively used for research and for diagnosis. To detect antigens on nitrocellulose sheets, radioactive and non-radioactive labels have been introduced in recent years (2, 3-11). Recently we reported the development of an alternative, highly sensitive bioluminescence-enhanced detection system for protein blotting (1). This method uses firefly (Photinus pyralis) bioluminescence (1, 12, 13) and the emitted light is measured by exposing sensitive photographic film. J. Clin. Chem. Clin. Biochem. /Vol. 26,1988 / No. 3 Light detection was performed with a photon counting camera system (Argus 100) kindly provided by Hamamatsu Photonics, D-8036 Herrsching,
Clinical Chemistry and Laboratory Medicine – de Gruyter
Published: Jan 1, 1988
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