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Salinity Effects on Ruppia maritima L. Cultured In Vitro

Salinity Effects on Ruppia maritima L. Cultured In Vitro Salinity effects were tested on rhizome growth and rooting of Ruppia maritima L. (Potamogetonaceae) cultured in vitro. In three serial 4 week experiments, production of new rhizome nodes was greatest in half strength Murashige and Skoog media prepared with distilled water (0%o) or 5%o artificial seawater, compared to growth in media made with 10, 15 and 20%o artificial seawater. In rooting trials, root production and growth required the presence of a carbon source and was greater in media made with 5 and 10%o artificial seawater. Longest root growth occurred in media where sodium bicarbonate was provided as the carbon source compared to the use of sucrose. Plants from rooting media were successfully acclimated ex vitro in concrete tanks with flowing seawater. Plants were also directly rooted ex vitro with 100% success. These results indicate that R. maritima can be rapidly propagated through in vitro culture. Introduction Loss of seagrass habitats due to coastal development and water quality degradation has led to the need for techniques to restore and create seagrass meadows (Lewis 1987). Much of the revegetation effort has been a failure due to a lack of scientific information on environmental requirements of transplant species (Fonseca et al. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Botanica Marina de Gruyter

Salinity Effects on Ruppia maritima L. Cultured In Vitro

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References (9)

Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
0006-8055
eISSN
1437-4323
DOI
10.1515/botm.1993.36.1.23
Publisher site
See Article on Publisher Site

Abstract

Salinity effects were tested on rhizome growth and rooting of Ruppia maritima L. (Potamogetonaceae) cultured in vitro. In three serial 4 week experiments, production of new rhizome nodes was greatest in half strength Murashige and Skoog media prepared with distilled water (0%o) or 5%o artificial seawater, compared to growth in media made with 10, 15 and 20%o artificial seawater. In rooting trials, root production and growth required the presence of a carbon source and was greater in media made with 5 and 10%o artificial seawater. Longest root growth occurred in media where sodium bicarbonate was provided as the carbon source compared to the use of sucrose. Plants from rooting media were successfully acclimated ex vitro in concrete tanks with flowing seawater. Plants were also directly rooted ex vitro with 100% success. These results indicate that R. maritima can be rapidly propagated through in vitro culture. Introduction Loss of seagrass habitats due to coastal development and water quality degradation has led to the need for techniques to restore and create seagrass meadows (Lewis 1987). Much of the revegetation effort has been a failure due to a lack of scientific information on environmental requirements of transplant species (Fonseca et al.

Journal

Botanica Marina – de Gruyter

Published: Jan 1, 1993

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