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T. Wallimann, H. Kuhn, G. Pelloni, D. Turner, H. Eppenberger (1977)
Localization of creatine kinase isoenzymes in myofibrils. II. Chicken heart muscleThe Journal of Cell Biology, 75
M. Tsuji, R. Mulkern, C. Cook, R. Meyers, D. Holtzman (1996)
Relative phosphocreatine and nucleoside triphosphate concentrations in cerebral gray and white matter measured in vivo by 31P nuclear magnetic resonanceBrain Research, 707
R. Wevers, C. Reutelingsperger, B. Dam, J. Soons (1982)
Mitochondrial creatine kinase (EC 2.7.3.2) in the brain.Clinica chimica acta; international journal of clinical chemistry, 119 3
T. Schnyder, M. Cyrklaff, K. Fuchs, T. Wallimann (1994)
Crystallization of mitochondrial creatine kinase on negatively charged lipid layers.Journal of structural biology, 112 2
Robert Haas, Clifford Korenfeld, Zhifang Zhang, Benjamin PerrymanQ, Dragos Roman, Arnold Straussn (1989)
Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase.The Journal of biological chemistry, 264 5
M. Beal (1998)
Mitochondrial dysfunction in neurodegenerative diseases.Biochimica et biophysica acta, 1366 1-2
A. Grace, Benjamin Perryman, Robert Roberts (1983)
Purification and characterization of human mitochondrial creatine kinase. A single enzyme form.The Journal of biological chemistry, 258 24
E. O'gorman, K. Fuchs, P. Tittmann, H. Gross, T. Wallimann (1997)
Crystalline mitochondrial inclusion bodies isolated from creatine depleted rat soleus muscle.Journal of cell science, 110 ( Pt 12)
R. Allen (1984)
Gel Electrophoresis and Isoelectric Focusing of Proteins
F. Studier, Rosenberg Ah, J. Dunn, Dubendorff Jw (1990)
Use of T7 RNA polymerase to direct expression of cloned genes.Methods in enzymology, 185
K. Miller, K. Sharer, J. Suhan, A. Koretsky (1997)
Expression of functional mitochondrial creatine kinase in liver of transgenic mice.The American journal of physiology, 272 4 Pt 1
M. Eder, K. Fritz-Wolf, W. Kabsch, T. Wallimann, U. Schlattner (2000)
Crystal structure of human ubiquitous mitochondrial creatine kinaseProteins: Structure, 39
Z. Khuchua, Wenning Qin, J. Boero, Judy Cheng, R. Payne, V. Saks, A. Strauss (1998)
Octamer Formation and Coupling of Cardiac Sarcomeric Mitochondrial Creatine Kinase Are Mediated by Charged N-terminal Residues*The Journal of Biological Chemistry, 273
G. Beutner, A. Rück, B. Riede, D. Brdiczka (1998)
Complexes between porin, hexokinase, mitochondrial creatine kinase and adenylate translocator display properties of the permeability transition pore. Implication for regulation of permeability transition by the kinases.Biochimica et biophysica acta, 1368 1
M. Wyss, P. James, J. Schlegel, T. Wallimann (1993)
Limited proteolysis of creatine kinase. Implications for three-dimensional structure and for conformational substrates.Biochemistry, 32 40
F. Rudolph, H. Fromm (1979)
Plotting methods for analyzing enzyme rate data.Methods in enzymology, 63
F. Sanger, S. Nicklen, A. Coulson (1977)
DNA sequencing with chain-terminating inhibitors.Proceedings of the National Academy of Sciences of the United States of America, 74 12
M. Wyss, J. Smeitink, R. Wevers, T. Wallimann (1992)
Mitochondrial creatine kinase: a key enzyme of aerobic energy metabolism.Biochimica et biophysica acta, 1102 2
N. Price, L. Stevens (1982)
Fundamentals of Enzymology
Z. Khuchua, Renée Ventura-Clapier, A. Kuznetsov, M. Grishin, Valdur Saks (1989)
Alterations in the creatine kinase system in the myocardium of cardiomyopathic hamsters.Biochemical and biophysical research communications, 165 2
T. Schnyder, A. Engel, A. Lustig, T. Wallimann (1988)
Native mitochondrial creatine kinase forms octameric structures. II. Characterization of dimers and octamers by ultracentrifugation, direct mass measurements by scanning transmission electron microscopy, and image analysis of single mitochondrial creatine kinase octamers.The Journal of biological chemistry, 263 32
K. Fritz-Wolf, T. Schnyder, T. Wallimann, W. Kabsch (1996)
Structure of mitochondrial creatine kinaseNature, 381
R. Furter, E. Furter-Graves, Theo Wallimann (1993)
Creatine kinase: the reactive cysteine is required for synergism but is nonessential for catalysis.Biochemistry, 32 27
R. Haas, A. Strauss (1990)
Separate nuclear genes encode sarcomere-specific and ubiquitous human mitochondrial creatine kinase isoenzymes.The Journal of biological chemistry, 265 12
U. Schlattner, T. Wallimann (2000)
Octamers of Mitochondrial Creatine Kinase Isoenzymes Differ in Stability and Membrane Binding*The Journal of Biological Chemistry, 275
M. Crompton (1999)
The mitochondrial permeability transition pore and its role in cell death.The Biochemical journal, 341 ( Pt 2)
S. Soboll, D. Brdiczka, D. Jahnke, A. Schmidt, U. Schlattner, S. Wendt, M. Wyss, T. Wallimann (1999)
Octamer-dimer transitions of mitochondrial creatine kinase in heart disease.Journal of molecular and cellular cardiology, 31 4
M. Wyss, R. Kaddurah-Daouk (2000)
Creatine and creatinine metabolism.Physiological reviews, 80 3
H. Blum, B. Deus, W. Gerok (1983)
Mitochondrial creatine kinase from human heart muscle: purification and characterization of the crystallized isoenzyme.Journal of biochemistry, 94 4
T. Wallimann, M. Wyss, D. Brdiczka, Klaas Nicolay, H. Eppenberger (1992)
Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the 'phosphocreatine circuit' for cellular energy homeostasis.The Biochemical journal, 281 ( Pt 1)
T. Wallimann, T. Schlösser, H. Eppenberger (1984)
Function of M-line-bound creatine kinase as intramyofibrillar ATP regenerator at the receiving end of the phosphorylcreatine shuttle in muscle.The Journal of biological chemistry, 259 8
M. Rojo, R. Hovius, R. Demel, T. Wallimann, H. Eppenberger, K. Nicolay (1991)
Interaction of mitochondrial creatine kinase with model membranes A monolayer studyFEBS Letters, 281
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
R. Furter, P. Kaldis, E. Furter-Graves, T. Schnyder, H. Eppenberger, T. Wallimann (1992)
Expression of active octameric chicken cardiac mitochondrial creatine kinase in Escherichia coli.The Biochemical journal, 288 ( Pt 3)
R. Pratt, Lea Vallis, C. Lim, W. Chisnall (1987)
MITOCHONDRIAL CREATINE KINASE IN CANCER PATIENTSPathology, 19
K. Fritz-Wolf, T. Schnyder, T. Wallimann, W. Kabsch (1997)
MITOCHONDRIAL CREATINE KINASE
Kanemitsu Fusae, K. Isami, Mi-Hee Jun, O. Tohru (1984)
Mitochondrial creatine kinase as a tumor-associated marker.Clinica Chimica Acta, 138
E. Milner-White, D. Watts (1971)
Inhibition of adenosine 5'-triphosphate-creatine phosphotransferase by substrate-anion complexes. Evidence for the transition-state organization of the catalytic site.The Biochemical journal, 122 5
W. Cleland (1967)
The statistical analysis of enzyme kinetic data.Advances in enzymology and related areas of molecular biology, 29
T. Frey, C. Mannella (2000)
The internal structure of mitochondria.Trends in biochemical sciences, 25 7
U. Schlattner, T. Wallimann (2000)
A Quantitative Approach to Membrane Binding of Human Ubiquitous Mitochondrial Creatine Kinase Using Surface Plasmon ResonanceJournal of Bioenergetics and Biomembranes, 32
M. Rojo, Ruud Hovius, Rudy Demel, Klaas Nicolay, Theo Wallimann (1991)
Mitochondrial creatine kinase mediates contact formation between mitochondrial membranes.The Journal of biological chemistry, 266 30
Martin Gross, Theo Wallimann (1993)
Kinetics of assembly and dissociation of the mitochondrial creatine kinase octamer. A fluorescence study.Biochemistry, 32 50
E. O'gorman, Giesela Beutner, M. Dolder, A. Koretsky, D. Brdiczka, T. Wallimann (1997)
The role of creatine kinase in inhibition of mitochondrial permeability transitionFEBS Letters, 414
Ursula Walterscheid-Müller, Siegmund Braun, Willi Salvenmoser, Georg Meffert, Otto Dapunt, Erich Gnaiger, Stephan Zierz, Raimund Margreiter, Markus Wyss (1997)
Purification and characterization of human sarcomeric mitochondrial creatine kinase.Journal of molecular and cellular cardiology, 29 3
Abstract The mitochondrial isoenzymes of creatine kinase (MtCK), ubiquitous uMtCK and sarcomeric sMtCK, are key enzymes of oxidative cellular energy metabolism and play an important role in human health and disease. Very little is known about uMtCK in general, or about sMtCK of human origin. Here we have heterologously expressed and purified both human MtCK isoenzymes to perform a biochemical, kinetic and structural characterization. Both isoenzymes occurred as octamers, which can dissociate into dimers. Distinct Stokes' radii of uMtCK and sMtCK in solution were indicative for conformational differences between these equally sized proteins. Both human MtCKs formed 2D-crystals on cardiolipin layers, which revealed further subtle differences in octamer structure and stability. Octameric human sMtCK displayed p 4 symmetry with lattice parameters of 145 Å, indicating a ‘flattening’ of the octamer on the phospholipid layer. pH optima and enzyme kinetic constants of the two human isoenzymes were significantly different. A pronounced substrate binding synergism ( K d > K m ) was observed for all substrates, but was most pronounced in the forward reaction (PCr production) of uMtCK and led to a significantly lower K m for creatine (1.01mM) and ATP (0.11mM) as compared to sMtCK (creatine, 7.31 mM; ATP, 0.68 mM).
Biological Chemistry – de Gruyter
Published: Nov 15, 2000
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