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S. Arold, F. Hoh, S. Domergue, C. Birck, M. Delsuc, M. Jullien, C. Dumas (2000)
Characterization and molecular basis of the oligomeric structure of HIV‐1 Nef proteinProtein Science, 9
Zhi‐Xin Wang (1995)
An exact mathematical expression for describing competitive binding of two different ligands to a protein moleculeFEBS Letters, 360
M. Sosa, R. Degasperi, Yanha Kim, F. Fazely, P. Sharma, R. Ruprecht (1991)
Serine phosphorylation-independent downregulation of cell-surface CD4 by nef.AIDS research and human retroviruses, 7 11
T. Swigut, N. Shohdy, J. Skowroński (2001)
Mechanism for down‐regulation of CD28 by NefThe EMBO Journal, 20
T. Stangler, R. Hartmann, D. Willbold, B. Koenig (2006)
Modern High Resolution NMR for the Study of Structure, Dynamics and Interactions of Biological MacromoleculesZeitschrift für Physikalische Chemie, 220
S. Grzesiek, A. Bax, G. Clore, A. Gronenborn, J. Hu, J. Kaufman, I. Palmer, S. Stahl, P. Wingfield (1996)
The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinaseNature Structural Biology, 3
K. Saksela (2004)
Therapeutic targeting of interactions between Nef and host cell proteins.Current drug targets. Immune, endocrine and metabolic disorders, 4 4
Stefan Arold, R. O'brien, Peet Franken, M. Strub, Francois Hoh, Christian Dumas, John Ladbury (1998)
RT loop flexibility enhances the specificity of Src family SH3 domains for HIV-1 Nef.Biochemistry, 37 42
T. Kohno, H. Kusunoki, Kazuki Sato, K. Wakamatsu (1998)
A new general method for the biosynthesis of stable isotope-enriched peptides using a decahistidine-tagged ubiquitin fusion system: An application to the production of mastoparan-X uniformly enriched with 15N and 15N/13CJournal of Biomolecular NMR, 12
M. Greenberg, A. Iafrate, J. Skowroński (1998)
The SH3 domain‐binding surface and an acidic motif in HIV‐1 Nef regulate trafficking of class I MHC complexesThe EMBO Journal, 17
S. Briggs, M. Sharkey, M. Stevenson, T. Smithgall (1997)
SH3-mediated Hck Tyrosine Kinase Activation and Fibroblast Transformation by the Nef Protein of HIV-1*The Journal of Biological Chemistry, 272
Lang Liu, N. Heveker, O. Fackler, S. Arold, S. Gall, K. Janvier, B. Peterlin, C. Dumas, O. Schwartz, S. Bénichou, R. Benarous (2000)
Mutation of a Conserved Residue (D123) Required for Oligomerization of Human Immunodeficiency Virus Type 1 Nef Protein Abolishes Interaction with Human Thioesterase and Results in Impairment of Nef Biological FunctionsJournal of Virology, 74
G. Bodenhausen, D. Ruben (1980)
Natural abundance nitrogen-15 NMR by enhanced heteronuclear spectroscopyChemical Physics Letters, 69
I. Bell, T. Schaefer, R. Trible, A. Amedee, T. Reinhart (2001)
Down-modulation of the costimulatory molecule, CD28, is a conserved activity of multiple SIV Nefs and is dependent on histidine 196 of Nef.Virology, 283 1
T. Schaefer, I. Bell, Melanie Pfeifer, M. Ghosh, R. Trible, Craig Fuller, C. Ashman, T. Reinhart (2002)
The conserved process of TCR/CD3 complex down-modulation by SIV Nef is mediated by the central core, not endocytic motifs.Virology, 302 1
I. Moarefi, M. LaFevre-Bernt, F. Sicheri, M. Huse, Chi-Hon Lee, J. Kuriyan, W. Miller (1997)
Activation of the Sire-family tyrosine kinase Hck by SH3 domain displacementNature, 385
R. Trible, Lori Emert-Sedlak, T. Smithgall (2006)
HIV-1 Nef Selectively Activates Src Family Kinases Hck, Lyn, and c-Src through Direct SH3 Domain Interaction*Journal of Biological Chemistry, 281
S. Arold, P. Franken, M. Strub, F. Hoh, S. Bénichou, R. Benarous, C. Dumas (1997)
The crystal structure of HIV-1 Nef protein bound to the Fyn kinase SH3 domain suggests a role for this complex in altered T cell receptor signaling.Structure, 5 10
F. Kirchhoff, T. Greenough, D. Brettler, J. Sullivan, R. Desrosiers (1995)
Absence of intact nef sequences in a long-term survivor with nonprogressive HIV-1 infectionThe New England Journal of Medicine, 332
H. Schmidt, S. Hoffmann, T. Tran, M. Stoldt, T. Stangler, Katja Wiesehan, D. Willbold (2007)
Solution structure of a Hck SH3 domain ligand complex reveals novel interaction modes.Journal of molecular biology, 365 5
F. Delaglio, S. Grzesiek, G. Vuister, G. Zhu, John Pfeifer, A. Bax (1995)
NMRPipe: A multidimensional spectral processing system based on UNIX pipesJournal of Biomolecular NMR, 6
Bruce Johnson (2004)
Using NMRView to visualize and analyze the NMR spectra of macromolecules.Methods in molecular biology, 278
K. Saksela, Genhong Cheng, David Baltimore (1995)
Proline‐rich (PxxP) motifs in HIV‐1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down‐regulation of CD4.The EMBO Journal, 14
N. Deacon, A. Tsykin, A. Solomon, K. Smith, M. Ludford-Menting, D. Hooker, D. McPhee, A. Greenway, Anne Ellett, C. Chatfield, V. Lawson, S. Crowe, A. Maerz, S. Sonza, J. Learmont, J. Sullivan, A. Cunningham, D. Dwyer, D. Dowton, J. Mills (1995)
Genomic Structure of an Attenuated Quasi Species of HIV-1 from a Blood Transfusion Donor and RecipientsScience, 270
R. Keller (2004)
The Computer Aided Resonance Assignment Tutorial
T. Tran, S. Hoffmann, Katja Wiesehan, E. Jonas, Cornelia Luge, Amine Aladağ, D. Willbold (2005)
Insights into human Lck SH3 domain binding specificity: different binding modes of artificial and native ligands.Biochemistry, 44 45
V. Stove, Inge Walle, E. Naessens, E. Coene, C. Stove, J. Plum, B. Verhasselt (2005)
Human Immunodeficiency Virus Nef Induces Rapid Internalization of the T-Cell Coreceptor CD8αβJournal of Virology, 79
Harry Kestier, D. Ringler, K. Mori, D. Panicali, P. Sehgal, M. Daniel, R. Desrosiers (1991)
Importance of the nef gene for maintenance of high virus loads and for development of AIDSCell, 65
M. Schindler, J. Münch, O. Kutsch, Hui Li, M. Santiago, F. Bibollet-Ruche, M. Müller-Trutwin, F. Novembre, M. Peeters, V. Courgnaud, E. Bailes, P. Roques, D. Sodora, G. Silvestri, P. Sharp, B. Hahn, F. Kirchhoff (2006)
Nef-Mediated Suppression of T Cell Activation Was Lost in a Lentiviral Lineage that Gave Rise to HIV-1Cell, 125
Renkema Gh, K. Saksela (2000)
Interactions of HIV-1 NEF with cellular signal transducing proteins.Frontiers in bioscience : a journal and virtual library, 5
C. Lepre, Jonathan Moore, Jeffrey Peng (2004)
Theory and Applications of NMR‐Based Screening in Pharmaceutical ResearchChemInform, 35
Huihui Ye, H. Choi, Jerrod Poe, T. Smithgall (2004)
Oligomerization is required for HIV-1 Nef-induced activation of the Src family protein-tyrosine kinase, Hck.Biochemistry, 43 50
H. Craig, M. Pandori, N. Riggs, D. Richman, J. Guatelli (1999)
Analysis of the SH3-binding region of HIV-1 nef: partial functional defects introduced by mutations in the polyproline helix and the hydrophobic pocket.Virology, 262 1
Chi-Hon Lee, K. Saksela, U. Mirza, B. Chait, J. Kuriyan (1996)
Crystal Structure of the Conserved Core of HIV-1 Nef Complexed with a Src Family SH3 DomainCell, 85
F. Kirchhoff, T. Greenough, D. Brettler, J. Sullivan, R. Desrosiers (1995)
Brief report: absence of intact nef sequences in a long-term survivor with nonprogressive HIV-1 infection.The New England journal of medicine, 332 4
Y. Fujii, K. Otake, Y. Fujita, N. Yamamoto, Y. Nagai, M. Tashiro, Akio Adachi (1996)
Clustered localization of oligomeric Nef protein of human immunodeficiency virus type 1 on the cell surfaceFEBS Letters, 395
C. Aiken, J. Konner, N. Landau, M. Lenburg, D. Trono (1994)
Nef induces CD4 endocytosis: Requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domainCell, 76
Kasia Hrecka, T. Swigut, M. Schindler, F. Kirchhoff, J. Skowroński (2005)
Nef Proteins from Diverse Groups of Primate Lentiviruses Downmodulate CXCR4 To Inhibit Migration to the Chemokine Stromal Derived Factor 1Journal of Virology, 79
A. Preußer, L. Briese, A. Baur, D. Willbold (2001)
Direct In Vitro Binding of Full-Length Human Immunodeficiency Virus Type 1 Nef Protein to CD4 Cytoplasmic DomainJournal of Virology, 75
M. Balter (1996)
HIV's Other Immune-System Targets: MacrophagesScience, 274
A. Mangasarian, V. Piguet, Jen-Kuei Wang, Yen-Liang Chen, D. Trono (1999)
Nef-Induced CD4 and Major Histocompatibility Complex Class I (MHC-I) Down-Regulation Are Governed by Distinct Determinants: N-Terminal Alpha Helix and Proline Repeat of Nef Selectively Regulate MHC-I TraffickingJournal of Virology, 73
Chi-Hon Lee, Benjamin Leung, M. Lemmon, Jie Zheng, D. Cowburn, J. Kuriyan, K. Saksela (1995)
A single amino acid in the SH3 domain of Hck determines its high affinity and specificity in binding to HIV‐1 Nef protein.The EMBO Journal, 14
R. Welker, H. Kottler, H. Kalbitzer, Hans-Georg Kräusslich (1996)
Human immunodeficiency virus type 1 Nef protein is incorporated into virus particles and specifically cleaved by the viral proteinase.Virology, 219 1
N. Kienzle, J. Freund, H. Kalbitzer, N. Mueller‐Lantzsch (1993)
Oligomerization of the Nef protein from human immunodeficiency virus (HIV) type 1.European journal of biochemistry, 214 2
Abstract We studied the interaction of the artificial 12-aa proline-rich peptide PD1 with the SH3 domain of the hematopoietic cell kinase Hck and the peptide's potency in competitively displacing HIV-1 Nef from the Hck SH3 domain. PD1 was obtained from a phage display screen and exhibits exceptional affinity for the Hck SH3 domain ( K d =0.23 μM). Competition experiments using NMR spectroscopy demonstrate that the peptide even displaces Nef from Hck SH3 and allow for estimation of the Nef-Hck SH3 dissociation constant ( K d =0.44 μM), the strongest SH3 ligand interaction known so far. Consequences of this study for novel antiviral concepts are discussed.
Biological Chemistry – de Gruyter
Published: Jun 1, 2007
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