USE OF A TETRAZOLIUM SALT FOR AN EASILY DISCERNIBLE SEPARATION OF SEROLOGICAL PHASES
Abstract
Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://jb.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ NOTES J. BACTERIOL. TABLE 1. Metabolism of glucose by resting cells of Listeria monocytogenes A4413* Growth medium Qo2(N)t ,umoles 02/ End products (pmole/pmole Armole Lactate Acetoin glucose) jAmleglucose) CO2 lucsePyruvate Carbon recovery BHI NS-3 (0.2% glucose) NS-3 (0.6% glucose) NS-4CA 444-773 594 375-475 193-315 Warburg vessel contents/3 ml: 1 mg of cell nitrogen, 10 ,moles of glucose, pH adjusted to 7.0 with 1 ml of 0.067 M potassium phosphate buffer, 0.1 ml of 2 N KOH in the center well, except in the case of CO,. The vessels were shaken 60 to 80 min at 36 C. t Qos (N) = sliters of 02 taken up per mg of bacterial nitrogen per hr. Range of values represents three to five replicate experiments except for NS-3 (0.2% glucose), which was run once. Downloaded from http://jb.asm.org/ on December 9, 2011 by deepdyve Brrucellosis in Man and Animals, rev. ed., Commonwealth Fund, New York, 1943; Rockenmacher, Proc. Soc. Exptl. Biol. Med. 71:99, 1949). On the other hand, strain Cornell grown in