SUBSTITUTION OF KNOWN COMPOUNDS FOR ASCITIC FLUID IN THE CULTIVATION OF BORRELIA VINCENTII
Abstract
Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://jb.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ EDWARD G. HAMPP' AND THOMAS A. NEVIN National Institute of Dental Research, National Institutes of Health, Public Health Service, U. S. Department of Health, Education, and Welfare, Bethesda, Mlaryland Received for publication December 22, 1958 The isolation and cultivation of the various MATERIALS AND METHODS members of the family Treponemataceae on artificial culture media have been complicated first by the need of an adequate base medium for growth, and secondly by the absolute requirement of the microorganisms for ascitic fluid, blood, plasma, serum, or serum products (Noguchi, 1912; Kast and Kolmer, 1940; Rosebury and Foley, 1942; Wichelhausen and WVichelhausen, 1942; Hampp, 1943, 1954; Little and SubbaRow, 1945; Fitzgerald and Hampp, 1952). Ascitic fluid is in most instances superior to all other materials used for this purpose, particularly for primary isolation of the spirochaetes (Hampp, 1943). Once the microorganisms are obtained in pure culture, many of the serum products may be substituted for ascitic fluid with success for maintenance of the strains. The lack of knowledge of the nature