STUDIES ON THE METABOLISM OF SHIGELLA IV. 3
Abstract
STUDIES ON THE METABOLISM OF SHIGELLA IV. 3 Nutritional Requirements of Shigella Flexneri Serotype Sylvia F. Pan , Robert B. Yee and Horace M. Gezon Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania ABSTRACT Pan, Sylvia F. (University of Pittsburgh, Pittsburgh, Pa.), Robert B. Yee, and Horace M. Gezon . Studies on the metabolism of Shigella . IV. Nutritional requirements of Shigella flexneri serotype 3. J. Bacteriol. 83: 61–65. 1962.—A study of the nutritional requirements of five strains of Shigella flexneri serotype 3 was carried out. Four of the strains could be cultured in a synthetic medium composed of a mineral salts glucose base, NH 4 H 2 PO 4 or an amino acid as the nitrogen source, and nicotinamide; the fifth one required the presence of both NH 4 H 2 PO 4 and an amino acid in the medium. Variation among the former strains in their nitrogen and vitamin requirements was observed. The least fastidious one produced maximal growth using the simple ammonium salt as its source of nitrogen and did not require the addition of nicotinamide. Two strains were moderately fastidious, producing better growth within 24 hr in the presence of l -aspartic acid instead of NH 4 H 2 PO 4 , and requiring nicotinamide as an accessory growth factor. The fourth strain was extremely fastidious, growing poorly or not at all in the synthetic medium. Incubation of large inocula of the fastidious strain in synthetic medium and over a prolonged period resulted in adaptation of the organism to the medium. Some of the adapted cultures grew readily in the presence of NH 4 H 2 PO 4 and did not require nicotinamide. Copyright © 1962, The Williams & Wilkins Company CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article J. Bacteriol. January 1962 vol. 83 no. 1 61-65 » Abstract PDF Classifications ARTICLE Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of JB Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Pan, S. F. Articles by Gezon, H. M. Search for related content PubMed PubMed citation Articles by Pan, S. F. Articles by Gezon, H. M. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue January 2012, volume 194, issue 1 Alert me to new issues of JB About JB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0021-9193 Online ISSN: 1098-5530 Copyright © 2011 by the American Society for Microbiology. For an alternate route to JB .asm.org, visit: http://intl- JB .asm.org | More Info» var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-5821458-9"); pageTracker._trackPageview();