Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Species Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems

Species Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two... Species Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems ▿ Ulrik Stenz Justesen 1 , * , Anette Holm 2 , Elisa Knudsen 1 , Line Bisgaard Andersen 2 , Thøger Gorm Jensen 1 , Michael Kemp 1 , Marianne Nielsine Skov 1 , Bente Gahrn-Hansen 1 and Jens Kjølseth Møller 2 1 Department of Clinical Microbiology, Odense University Hospital, Odense 2 Department of Clinical Microbiology, Vejle Hospital, Vejle, Denmark ABSTRACT We compared two matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria ( n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Microbiology American Society For Microbiology

Species Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems

Download PDF

Species Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems

Justesen, Ulrik Stenz; Holm, Anette; Knudsen, Elisa; Andersen, Line Bisgaard; Jensen, Thøger Gorm; Kemp, Michael; Skov, Marianne Nielsine; Gahrn-Hansen, Bente; Møller, Jens Kjølseth
Journal of Clinical Microbiology , Volume 49 (12): 4314 – Dec 1, 2011

Abstract

Species Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems ▿ Ulrik Stenz Justesen 1 , * , Anette Holm 2 , Elisa Knudsen 1 , Line Bisgaard Andersen 2 , Thøger Gorm Jensen 1 , Michael Kemp 1 , Marianne Nielsine Skov 1 , Bente Gahrn-Hansen 1 and Jens Kjølseth Møller 2 1 Department of Clinical Microbiology, Odense University Hospital, Odense 2 Department of Clinical Microbiology, Vejle Hospital, Vejle, Denmark ABSTRACT We compared two matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria ( n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria.

Loading next page...
 
/lp/american-society-for-microbiology/species-identification-of-clinical-isolates-of-anaerobic-bacteria-a-r46CfEzxka

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
American Society For Microbiology
Copyright
Copyright © 2011 by the American society for Microbiology.
ISSN
0095-1137
eISSN
1098-660X
DOI
10.1128/JCM.05788-11
pmid
21998433
Publisher site
See Article on Publisher Site

Abstract

Species Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems ▿ Ulrik Stenz Justesen 1 , * , Anette Holm 2 , Elisa Knudsen 1 , Line Bisgaard Andersen 2 , Thøger Gorm Jensen 1 , Michael Kemp 1 , Marianne Nielsine Skov 1 , Bente Gahrn-Hansen 1 and Jens Kjølseth Møller 2 1 Department of Clinical Microbiology, Odense University Hospital, Odense 2 Department of Clinical Microbiology, Vejle Hospital, Vejle, Denmark ABSTRACT We compared two matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria ( n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria.

Journal

Journal of Clinical Microbiology – American Society For Microbiology

Published: Dec 1, 2011

References

  • Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory
    Bizzini
  • Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification
    Bizzini
  • Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level
    Cherkaoui
  • EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences
    Chun
  • How far should a clinical laboratory go in identifying anaerobic isolates, and who should pay?
    Citron
  • Interpretive criteria for identification of bacteria and fungi by DNA target sequencing
    Clinical and Laboratory Standards Institute
  • Evaluation of the Rapid ID 32A system for identification of anaerobic Gram-negative bacilli, excluding the Bacteroides fragilis group
    Downes
  • 16S rRNA gene sequencing in routine identification of anaerobic bacteria isolated from blood cultures
    Justesen
  • Preliminary results from a comparison of two matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry (MALDI-TOF) systems with 16S rRNA gene sequencing for identification of anaerobic bacteria to the species level
    Justesen
  • The EUCAST Mueller-Hinton fastidious agar for antimicrobial susceptibility testing of anaerobic bacteria?
    Justesen
  • Evaluation of the Vitek 2 ANC card for identification of clinical isolates of anaerobic bacteria
    Lee
  • Population genetic analysis of Propionibacterium acnes identifies a subpopulation and epidemic clones associated with acne
    Lomholt
  • Species identification of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry
    for the ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria
  • A revolution in the identification of pathogens in clinical laboratories
    Nassif
  • Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    Seng
  • High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories
    van Veen
  • Identification of Gram-positive anaerobic cocci by MALDI-TOF mass spectrometry
    Veloo
  • Comparison of two matrix-assisted laser desorption ionisation-time of flight mass spectrometry methods for the identification of clinically relevant anaerobic bacteria
    Veloo
  • The identification of anaerobic bacteria using MALDI-TOF MS
    Veloo