Subscribe to thousands of academic journals for just $40/month
Read and share the articles you need for your research, all in one place.

Separation of a New Deoxyribonucleic Acid Polymerase from Vaccinia-infected HeLa Cells

Journal of Virology , Volume 4 (1): 15 – Jul 1, 1969

Details

Publisher
American Society for Microbiology
Copyright
Copyright © 1969 by the American society for Microbiology.
ISSN
0022-538X
eISSN
1098-5514
Publisher site
See Article on Publisher Site

Preview Only

Expand Tray Hide Tray

Separation of a New Deoxyribonucleic Acid Polymerase from Vaccinia-infected HeLa Cells

Abstract

Separation of a New Deoxyribonucleic Acid Polymerase from Vaccinia-infected HeLa Cells K. I. Berns , C. Silverman and A. Weissbach 1 Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014 ABSTRACT HeLa cell deoxyribonucleic acid (DNA) polymerase was purified about 100-fold by sequential column chromatography on phosphocellulose, hydroxylapatite, and Bio Rex 70. A new form of DNA polymerase found in vaccinia virus-infected cells was separated from HeLa DNA polymerase by chromatography on diethylamino-ethyl cellulose. The new form was also purified approximately 100-fold in the same manner as the HeLa DNA polymerase. In addition to chromatographic differences, the two enzymes differed with regard to primer response, relative activity at high p H, inactivation by heat and p -chloromercuribenzoate, and inhibition by vaccinia antiserum.
Loading next page...

Preview Only. This article cannot be rented because we do not currently have permission from the publisher.

 
/lp/american-society-for-microbiology/separation-of-a-new-deoxyribonucleic-acid-polymerase-from-vaccinia-Lcm8Qv3SUM