Rhb1 Regulates the Expression of Secreted Aspartic Protease 2 through the TOR Signaling Pathway in Candida albicans Yu-Ting Chen a , Chia-Ying Lin a , Pei-Wen Tsai a , Cheng-Yao Yang b , Wen-Ping Hsieh c and Chung-Yu Lan a , d a Institute of Molecular and Cellular Biology c Institute of Statistics d Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan b Division of Animal Medicine, Animal Technology Institute Taiwan, Chunan, Miaoli, Taiwan ABSTRACT Candida albicans is a major fungal pathogen in humans. In C. albicans , secreted aspartyl protease 2 (Sap2) is the most highly expressed secreted aspartic protease in vitro and is a virulence factor. Recent research links the small GTPase Rhb1 to C. albicans target of rapamycin (TOR) signaling in response to nitrogen availability. The results of this study show that Rhb1 is related to cell growth through the control of SAP2 expression when protein is the major nitrogen source. This process involves various components of the TOR signaling pathway, including Tor1 kinase and its downstream effectors. TOR signaling not only controls SAP2 transcription but also affects Sap2 protein levels, possibly through general amino acid control. DNA microarray analysis identifies other target genes downstream of Rhb1 in addition to SAP2 . These findings provide new insight into nutrients, Rhb1-TOR signaling, and expression of C. albicans virulence factor.
Rhb1 Regulates the Expression of Secreted Aspartic Protease 2 through the TOR Signaling Pathway in Candida albicans
Abstract
Rhb1 Regulates the Expression of Secreted Aspartic Protease 2 through the TOR Signaling Pathway in Candida albicans Yu-Ting Chen a , Chia-Ying Lin a , Pei-Wen Tsai a , Cheng-Yao Yang b , Wen-Ping Hsieh c and Chung-Yu Lan a , d a Institute of Molecular and Cellular Biology c Institute of Statistics d Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan b Division of Animal Medicine, Animal Technology Institute Taiwan, Chunan, Miaoli, Taiwan ABSTRACT Candida albicans is a major fungal pathogen in humans. In C. albicans , secreted aspartyl protease 2 (Sap2) is the most highly expressed secreted aspartic protease in vitro and is a virulence factor. Recent research links the small GTPase Rhb1 to C. albicans target of rapamycin (TOR) signaling in response to nitrogen availability. The results of this study show that Rhb1 is related to cell growth through the control of SAP2 expression when protein is the major nitrogen source. This process involves various components of the TOR signaling pathway, including Tor1 kinase and its downstream effectors. TOR signaling not only controls SAP2 transcription but also affects Sap2 protein levels, possibly through general amino acid control. DNA microarray analysis identifies other target genes downstream of Rhb1 in addition to SAP2 . These findings provide new insight into nutrients, Rhb1-TOR signaling, and expression of C. albicans virulence factor.Preview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Rhb1 Regulates the Expression of Secreted Aspartic Protease 2 through the TOR Signaling Pathway in Candida albicans
Chen, Yu-Ting; Lin, Chia-Ying; Tsai, Pei-Wen; Yang, Cheng-Yao; Hsieh, Wen-Ping; Lan, Chung-Yu
Follow Eukaryotic Cell
, Volume 11 (2): 168
American Society For Microbiology – Feb 1, 2012
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