Review of frequency-pulsed electron-capture gas-liquid chromatography studies of diarrheal diseases caused by members of the family Enterobacteriaceae, Clostridium difficile, and rotavirus.
AbstractReceive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), moreÂ» Information about commercial reprint orders: http://jcm.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 1986, p. 687-691 0095-1137/86/110687-05$02.00/0 Copyright Â© 1986, American Society for Microbiology Vol. 24, No. 5 Review of Frequency-Pulsed Electron-Capture Gas-Liquid Chromatography Studies of Diarrheal Diseases Caused by Members of the Family Enterobacteriaceae, Clostridium difcile, and Rotavirus JOHN B. BROOKS Analytical Chemistry Laboratory, Meningitis and Special Pathogens Branch, Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia 30333 INTRODUCTION FPEC detector. The types of compounds detected during the study (3, 4, 8)were carboxylic acids, hydroxy acids, alcohols, and amines. The carboxylic acids were extracted as previously described (4) with nanograde chloroform from acidified watery diarrheal stools that had first been centrifuged to remove particulate matter. Formed stools were first made watery by the addition of 9 ml of distilled water to 1 ml of stool; then they were processed in the same way as watery diarrheal stools. The carboxylic acids were derivatized as previously described (1, 4) with trichloroethanol (TCE) and heptafluorobutyric anhydride (HFBA). TCE derivatizes only the carboxylic