Rapid fixed-time assay for penicillinase.
Abstract
CONTENT ALERTS Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://jb.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ JOURNAL OF BACTERIOLOGY, Apr. 1968, p. 1493-1494 Copyright © 1968 American Society for Microbiology Vol. 95, No. 4 Prinited in U.S.A. Itistitute of Microbiology, MICHAEL G. SARGENT Rutgers, The State University, New Brunswick, New Jersey 08903 Received for publication 29 November 1967 Many methods of assaying penicillinase activity have been devised (N. Citri and M. R. Pollock, Advan. Enzymol. 28:237, 1966; N. Citri, Methods Med. Res., 10:221, 1964; J. M. T. HamiltonMiller et al., J. Pharm. Pharmacol. 15:81, 1963; D. A. Wolfe and M. Hamberger, J. Lab. Clin. Med. 59:469, 1962). These have included colorimetric determinations of acid or alkali production, manometric techniques, direct spectrophotometry, and iodometric assays of penicillin degradation products. Virtually all of these either involve time-consuming rate measurements or have inadequate sensitivity. In this communication, a simple and rapid fixed-time assay is described that can be adapted for use with most types of colorimetric equipment. The method is evolved from that of C. J. Perret (Nature 174:1012, 1954) in which iodine strongly buffered