<h2>LETTER</h2> Campylobacter jejuni ATCC 33560 (CCM 6214) is the quality-control (QC) strain for the antimicrobial susceptibility testing of Campylobacter spp. ( 1 , 6 ). We have utilized the C. jejuni ATCC 33560 strain in antimicrobial susceptibility testing and in studies to explore antimicrobial resistance mechanisms. In our previous study, C. jejuni ATCC 33560 was a parental strain to induce ciprofloxacin-resistant mutants ( 4 ). According to sequence analysis, these mutants did not have point mutations in the quinolone resistance-determining region (QRDR) of the gyrA gene or mutations in the promoter region of cmeABC that encodes a multidrug efflux pump. However, the ciprofloxacin MICs were increased (8 to 16 μg/ml [ 4 ]; ATCC 33560, 0.12 to 0.5 μg/ml [ 6 ]), indicating the presence of unknown mutations. As the multidrug efflux pump CmeABC is repressed by CmeR ( 5 ), we included it in our further characterization of the mutants. Unexpectedly, the cmeR gene of C. jejuni ATCC 33560 parental strain, as well as its resistant mutants, contained a single A nucleotide deletion in the 8-nucleotide A repeat unit (bases 463 to 470 of cmeR , locus tag Cj0368c in NCTC 11168), causing a shift out-of-frame and
Quality Control Strain Campylobacter jejuni ATCC 33560 Contains a Frameshift Mutation in the CmeR Regulator
Abstract
<h2>LETTER</h2> Campylobacter jejuni ATCC 33560 (CCM 6214) is the quality-control (QC) strain for the antimicrobial susceptibility testing of Campylobacter spp. ( 1 , 6 ). We have utilized the C. jejuni ATCC 33560 strain in antimicrobial susceptibility testing and in studies to explore antimicrobial resistance mechanisms. In our previous study, C. jejuni ATCC 33560 was a parental strain to induce ciprofloxacin-resistant mutants ( 4 ). According to sequence analysis, these mutants did not have point mutations in the quinolone resistance-determining region (QRDR) of the gyrA gene or mutations in the promoter region of cmeABC that encodes a multidrug efflux pump. However, the ciprofloxacin MICs were increased (8 to 16 μg/ml [ 4 ]; ATCC 33560, 0.12 to 0.5 μg/ml [ 6 ]), indicating the presence of unknown mutations. As the multidrug efflux pump CmeABC is repressed by CmeR ( 5 ), we included it in our further characterization of the mutants. Unexpectedly, the cmeR gene of C. jejuni ATCC 33560 parental strain, as well as its resistant mutants, contained a single A nucleotide deletion in the 8-nucleotide A repeat unit (bases 463 to 470 of cmeR , locus tag Cj0368c in NCTC 11168), causing a shift out-of-frame andPreview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Quality Control Strain Campylobacter jejuni ATCC 33560 Contains a Frameshift Mutation in the CmeR Regulator
Hyytiäinen, Heidi; Hänninen, Marja-Liisa
Follow Antimicrobial Agents and Chemotherapy
, Volume 56 (2): 1148
American Society For Microbiology – Feb 1, 2012
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