Abstract

Photodynamic Action of Proflavine on Coliphage T3 I. Kinetics of Inactivation Heman Witmer and Dean Fraser 1 Microbiology Department, Indiana University, Bloomington, Indiana 47401 ABSTRACT The photodynamic inactivation of coliphage T3 was studied over a wide range of concentrations of the dye proflavine. With 2 × 10 7 phage/ml, two modes of inactivation were observed. Between 0.25 and 12 to 13 μg/ml, inactivation was biphasic. There was an initial first-order inactivation (R×1) which became temporally associated with an apparently multiorder process (R×2) at higher light doses. Dye concentrations above 12 to 13 μg/ml showed only two-target inactivation curves (R×3), except at high dye concentrations where processes kinetically identical to R×1 and R×2 reappeared. R×2 showed a normal rectangular hyperbolic saturation curve but R×1 and R×3 appeared to saturate prematurely. The saturation behavior of R×1 and R×2 was independent of phage concentration, but R×3 was lost at phage titers above 2 × 10 7 /ml. No dark inactivation was seen with R×1 and R×2 subsequent to a period of illumination. With R×3, an exponential dark inactivation was seen for at least 1 hr after a period of illumination. The dye-phage system equilibrated immediately, at any temperature, at proflavine concentrations where R×1 and R×2 occurred. With R×3, prolonged equilibration times were necessary. Moreover, there was a temperature effect. The rate of inactivation at equilibrium was temperature-dependent, whereas the initial rate at which equilibrium was approached was essentially temperature-independent. Copyright © 1971 American Society for Microbiology CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article J. Virol. March 1971 vol. 7 no. 3 314-318 » Abstract PDF Classifications Bacterial Viruses Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of JVI Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Witmer, H. Articles by Fraser, D. Search for related content PubMed PubMed citation Articles by Witmer, H. Articles by Fraser, D. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue January 2012, volume 86, issue 1 Spotlights in the Current Issue Two Xenotropic Murine Leukemia Virus Parents Breaking the Entry Targeting Barrier Complex Morphology and Dynamic Development of Poliovirus Membranous Replication Structures Revealed A Staining Artifact Explains Apparent Varicella-Zoster Virus Protein Expression in Neurons Recent Mumps Outbreaks Are Not Caused by Immune Escape Alert me to new issues of JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2011 by the American Society for Microbiology. For an alternate route to JVI .asm.org, visit: http://intl- JVI .asm.org | More Info» var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-5821458-1"); pageTracker._trackPageview();