Pathogenic Potential of Campylobacter ureolyticus Jose A. Burgos-Portugal a , Nadeem O. Kaakoush a , Mark J. Raftery b and Hazel M. Mitchell a a School of Biotechnology and Biomolecular Sciences, The University of New South Wales b Bioanalytical Mass Spectrometry Facility, The University of New South Wales, Sydney, Australia S. R. Blanke , Editor ABSTRACT The recent detection and isolation of the aflagellate Campylobacter ureolyticus (previously known as Bacteroides ureolyticus ) from intestinal biopsy specimens and fecal samples of children with newly diagnosed Crohn's disease led us to investigate the pathogenic potential of this bacterium. Adherence and gentamicin protection assays were employed to quantify the levels of adherence to and invasion into host cells. C. ureolyticus UNSWCD was able to adhere to the Caco-2 intestinal epithelial cell line with a value of 5.341% ± 0.74% but was not able to invade the Caco-2 cells. The addition of two proinflammatory cytokines, tumor necrosis factor alpha (TNF-α) and gamma interferon (IFN-γ), to the cell line did not affect attachment or invasion, with attachment levels being 4.156% ± 0.61% ( P = 0.270) for TNF-α and 6.472% ± 0.61% ( P = 0.235) for IFN-γ. Scanning electron microscopy visually confirmed attachment and revealed that C. ureolyticus UNSWCD colonizes and adheres to intestinal cells, inducing cellular damage and microvillus degradation. Purification and identification of the C. ureolyticus UNSWCD secretome detected a total of 111 proteins, from which 29 were bioinformatically predicted to be secretory proteins. Functional classification revealed three putative virulence and colonization factors: the surface antigen CjaA, an outer membrane fibronectin binding protein, and an S-layer RTX toxin. These results suggest that C. ureolyticus has the potential to be a pathogen of the gastrointestinal tract.
Pathogenic Potential of Campylobacter ureolyticus
Abstract
Pathogenic Potential of Campylobacter ureolyticus Jose A. Burgos-Portugal a , Nadeem O. Kaakoush a , Mark J. Raftery b and Hazel M. Mitchell a a School of Biotechnology and Biomolecular Sciences, The University of New South Wales b Bioanalytical Mass Spectrometry Facility, The University of New South Wales, Sydney, Australia S. R. Blanke , Editor ABSTRACT The recent detection and isolation of the aflagellate Campylobacter ureolyticus (previously known as Bacteroides ureolyticus ) from intestinal biopsy specimens and fecal samples of children with newly diagnosed Crohn's disease led us to investigate the pathogenic potential of this bacterium. Adherence and gentamicin protection assays were employed to quantify the levels of adherence to and invasion into host cells. C. ureolyticus UNSWCD was able to adhere to the Caco-2 intestinal epithelial cell line with a value of 5.341% ± 0.74% but was not able to invade the Caco-2 cells. The addition of two proinflammatory cytokines, tumor necrosis factor alpha (TNF-α) and gamma interferon (IFN-γ), to the cell line did not affect attachment or invasion, with attachment levels being 4.156% ± 0.61% ( P = 0.270) for TNF-α and 6.472% ± 0.61% ( P = 0.235) for IFN-γ. Scanning electron microscopy visually confirmed attachment and revealed that C. ureolyticus UNSWCD colonizes and adheres to intestinal cells, inducing cellular damage and microvillus degradation. Purification and identification of the C. ureolyticus UNSWCD secretome detected a total of 111 proteins, from which 29 were bioinformatically predicted to be secretory proteins. Functional classification revealed three putative virulence and colonization factors: the surface antigen CjaA, an outer membrane fibronectin binding protein, and an S-layer RTX toxin. These results suggest that C. ureolyticus has the potential to be a pathogen of the gastrointestinal tract.Preview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Pathogenic Potential of Campylobacter ureolyticus
Burgos-Portugal, Jose A.; Kaakoush, Nadeem O.; Raftery, Mark J.; Mitchell, Hazel M.
Follow Infection and Immunity
, Volume 80 (2): 883
American Society For Microbiology – Feb 1, 2012
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