Novel Microsphere-Based Method for Detection and Typing of 46 Mucosal Human Papillomavirus Types Vanessa Zubach a , Gerry Smart b , Samuel Ratnam a , c and Alberto Severini a , d a National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba b Cadham Provincial Laboratory, Winnipeg, Manitoba c Faculty of Medicine, Memorial University, St. John's, Newfoundland and Labrador d Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada ABSTRACT We have developed a novel microsphere-based genotyping method for 46 mucosal human papillomavirus (HPV) types. HPV DNA was amplified by PCR using general primers and typed by hybridization to HPV type-specific probes coupled to sortable microspheres based on the Luminex xMAP technology. Hybridization to each probe was specific for each HPV type without cross-hybridization and sensitive enough to allow typing of HPV contained in clinical specimens. The method was validated with direct sequencing and the Roche Linear Array genotyping method.
Novel Microsphere-Based Method for Detection and Typing of 46 Mucosal Human Papillomavirus Types
Abstract
Novel Microsphere-Based Method for Detection and Typing of 46 Mucosal Human Papillomavirus Types Vanessa Zubach a , Gerry Smart b , Samuel Ratnam a , c and Alberto Severini a , d a National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba b Cadham Provincial Laboratory, Winnipeg, Manitoba c Faculty of Medicine, Memorial University, St. John's, Newfoundland and Labrador d Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada ABSTRACT We have developed a novel microsphere-based genotyping method for 46 mucosal human papillomavirus (HPV) types. HPV DNA was amplified by PCR using general primers and typed by hybridization to HPV type-specific probes coupled to sortable microspheres based on the Luminex xMAP technology. Hybridization to each probe was specific for each HPV type without cross-hybridization and sensitive enough to allow typing of HPV contained in clinical specimens. The method was validated with direct sequencing and the Roche Linear Array genotyping method.Preview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Novel Microsphere-Based Method for Detection and Typing of 46 Mucosal Human Papillomavirus Types
Zubach, Vanessa; Smart, Gerry; Ratnam, Samuel; Severini, Alberto
Follow Journal of Clinical Microbiology
, Volume 50 (2): 460
American Society For Microbiology – Feb 1, 2012
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