<h2>LETTER</h2> In 2006, xenotropic murine leukemia virus (MLV)-related gammaretrovirus (XMRV) was isolated from prostate cancer tissue ( 9 ). However, subsequent studies have yielded conflicting and controversial results, with widespread detection of XMRV suggested to be the result of contamination with mouse DNA ( 3 ). While recent work has focused on PCR-based targeted detection of XMRV ( 10 ), the advent of next-generation sequencing (NGS) means that it is now possible to interrogate the entire genomes and transcriptomes of human samples for the unique genomic signatures of thousands of viruses ( 1 , 5 , 6 ). With this in mind, we analyzed whole-genome (DNA-Seq) and transcriptome (RNA-Seq) data from 9 human prostate tumors (6 primary and 3 metastatic), 3 prostate tumor-derived murine xenografts, and 1 benign tissue sample from a pelvic lymph node. The xenograft tumors carried significant amounts of host mouse tissue, thereby acting as positive controls. We mapped all nonhuman RNA-Seq reads to a custom database of 3,932 viral genomes and 1,387 microbial genomes downloaded from the National Center for Biotechnology Information RefSeq database (July 2011) ( 7 ) and filtered for reads mapping specifically to MLVs, the family that includes XMRV. Murine genomes
Next-Generation Sequencing of Prostate Tumors Provides Independent Evidence of Xenotropic Murine Leukemia Virus-Related Gammaretrovirus Contamination
Abstract
<h2>LETTER</h2> In 2006, xenotropic murine leukemia virus (MLV)-related gammaretrovirus (XMRV) was isolated from prostate cancer tissue ( 9 ). However, subsequent studies have yielded conflicting and controversial results, with widespread detection of XMRV suggested to be the result of contamination with mouse DNA ( 3 ). While recent work has focused on PCR-based targeted detection of XMRV ( 10 ), the advent of next-generation sequencing (NGS) means that it is now possible to interrogate the entire genomes and transcriptomes of human samples for the unique genomic signatures of thousands of viruses ( 1 , 5 , 6 ). With this in mind, we analyzed whole-genome (DNA-Seq) and transcriptome (RNA-Seq) data from 9 human prostate tumors (6 primary and 3 metastatic), 3 prostate tumor-derived murine xenografts, and 1 benign tissue sample from a pelvic lymph node. The xenograft tumors carried significant amounts of host mouse tissue, thereby acting as positive controls. We mapped all nonhuman RNA-Seq reads to a custom database of 3,932 viral genomes and 1,387 microbial genomes downloaded from the National Center for Biotechnology Information RefSeq database (July 2011) ( 7 ) and filtered for reads mapping specifically to MLVs, the family that includes XMRV. Murine genomesPreview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Next-Generation Sequencing of Prostate Tumors Provides Independent Evidence of Xenotropic Murine Leukemia Virus-Related Gammaretrovirus Contamination
Mo, Fan; Wyatt, Alexander W.; Wu, Chunxiao; Lapuk, Anna V.; Marra, Marco A.; Gleave, Martin E.; Volik, Stanislav V.; Collins, Colin C.
Follow Journal of Clinical Microbiology
, Volume 50 (2): 536
American Society For Microbiology – Feb 1, 2012
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