Abstract

Long-Term Preservation of Fungal Isolates in Commercially Prepared Cryogenic Microbank Vials A. Espinel-Ingroff 1 , * , D. Montero 2 and E. Martin-Mazuelos 2 1 VCU Medical Center, Richmond, Virginia 2 Valme University Hospital, Seville, Spain ABSTRACT Since 1994, 6,198 yeasts and 391 molds belonging to 25 and 37 species, respectively, were stored in Microbank cryogenic vials at ≥−130°C in liquid nitrogen and at −70°C in a freezer. All of the isolates, with the exception of 45 yeasts and 15 dermatophytes, were recovered from both storage temperatures. Good reproducibility was demonstrated for amphotericin B, fluconazole, and voriconazole MICs determined for random isolates.