Abstract

CONTENT ALERTS Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://jb.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ JOURNAL OF BACrERIOLOGY, Mar. 1993, p. 1548-1549 Vol. 175, No. 5 0021-9193/93/051548-02$02.00/0 Copyright © 1993, American Society for Microbiology E. COLI MAP Location of the rpoN Gene on the Physical Map of Escherichia coli MIKE MERRICK,"* DAVID H. A. JONES,2 AND CHRISTOPHER M. THOMAS2 AFRC Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ,' and School of Biological Sciences, University of Birmingham, Birmingham B15 217,2 United Kingdom Downloaded from http://jb.asm.org/ on December 6, 2011 by deepdyve The Escherichia coli rpoN (glnfl gene encodes the novel RNA polymerase sigma factor &e' (4, 5, 9). During the isolation of the homologous gene from Klebsiella pneumoniae, we purified R-prime DNA carrying K pneumoniae rpoN from an E. coli K-12 strain and cloned the gene by complementation (10). Two classes of plasmid were obtained, one (pMM17) with a 1.8-kb ClaI insert carrying K pneumoniae rpoN and one (pMM18) with a 4.6-kb ClaI insert carrying E. coli rpoN. Both plasmids have since been used in a number of laboratories to characterize