Involvement of T6 Pili in Biofilm Formation by Serotype M6 Streptococcus pyogenes Keiji Richard Kimura a , Masanobu Nakata a , Tomoko Sumitomo a , Bernd Kreikemeyer b , Andreas Podbielski b , Yutaka Terao a and Shigetada Kawabata a a Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan b Institute of Medical Microbiology, Virology and Hygiene, University of Rostock, Rostock, Germany ABSTRACT The group A streptococcus (GAS) Streptococcus pyogenes is known to cause self-limiting purulent infections in humans. The role of GAS pili in host cell adhesion and biofilm formation is likely fundamental in early colonization. Pilus genes are found in the FCT ( f ibronectin-binding protein, c ollagen-binding protein, and t rypsin-resistant antigen) genomic region, which has been classified into nine subtypes based on the diversity of gene content and nucleotide sequence. Several epidemiological studies have indicated that FCT type 1 strains, including serotype M6, produce large amounts of monospecies biofilm in vitro . We examined the direct involvement of pili in biofilm formation by serotype M6 clinical isolates. In the majority of tested strains, deletion of the tee6 gene encoding pilus shaft protein T6 compromised the ability to form biofilm on an abiotic surface. Deletion of the fctX and srtB genes, which encode pilus ancillary protein and class C pilus-associated sortase, respectively, also decreased biofilm formation by a representative strain. Unexpectedly, these mutant strains showed increased bacterial aggregation compared with that of the wild-type strain. When the entire FCT type 1 pilus region was ectopically expressed in serotype M1 strain SF370, biofilm formation was promoted and autoaggregation was inhibited. These findings indicate that assembled FCT type 1 pili contribute to biofilm formation and also function as attenuators of bacterial aggregation. Taken together, our results show the potential role of FCT type 1 pili in the pathogenesis of GAS infections.
Involvement of T6 Pili in Biofilm Formation by Serotype M6 Streptococcus pyogenes
Abstract
Involvement of T6 Pili in Biofilm Formation by Serotype M6 Streptococcus pyogenes Keiji Richard Kimura a , Masanobu Nakata a , Tomoko Sumitomo a , Bernd Kreikemeyer b , Andreas Podbielski b , Yutaka Terao a and Shigetada Kawabata a a Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan b Institute of Medical Microbiology, Virology and Hygiene, University of Rostock, Rostock, Germany ABSTRACT The group A streptococcus (GAS) Streptococcus pyogenes is known to cause self-limiting purulent infections in humans. The role of GAS pili in host cell adhesion and biofilm formation is likely fundamental in early colonization. Pilus genes are found in the FCT ( f ibronectin-binding protein, c ollagen-binding protein, and t rypsin-resistant antigen) genomic region, which has been classified into nine subtypes based on the diversity of gene content and nucleotide sequence. Several epidemiological studies have indicated that FCT type 1 strains, including serotype M6, produce large amounts of monospecies biofilm in vitro . We examined the direct involvement of pili in biofilm formation by serotype M6 clinical isolates. In the majority of tested strains, deletion of the tee6 gene encoding pilus shaft protein T6 compromised the ability to form biofilm on an abiotic surface. Deletion of the fctX and srtB genes, which encode pilus ancillary protein and class C pilus-associated sortase, respectively, also decreased biofilm formation by a representative strain. Unexpectedly, these mutant strains showed increased bacterial aggregation compared with that of the wild-type strain. When the entire FCT type 1 pilus region was ectopically expressed in serotype M1 strain SF370, biofilm formation was promoted and autoaggregation was inhibited. These findings indicate that assembled FCT type 1 pili contribute to biofilm formation and also function as attenuators of bacterial aggregation. Taken together, our results show the potential role of FCT type 1 pili in the pathogenesis of GAS infections.Preview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Involvement of T6 Pili in Biofilm Formation by Serotype M6 Streptococcus pyogenes
Kimura, Keiji Richard; Nakata, Masanobu; Sumitomo, Tomoko; Kreikemeyer, Bernd; Podbielski, Andreas; Terao, Yutaka; Kawabata, Shigetada
Follow Journal of Bacteriology
, Volume 194 (4): 804
American Society For Microbiology – Feb 15, 2012
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