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Feasibility of Screening for Penicillinase-Producing Neisseria gonorrhoeae from Primary Culture Plates by Using a Rapid Microacidometric Test

Feasibility of Screening for Penicillinase-Producing Neisseria gonorrhoeae from Primary Culture Plates by Using a Rapid Microacidometric Test

Abstract

Feasibility of Screening for Penicillinase-Producing Neisseria gonorrhoeae from Primary Culture Plates by Using a Rapid Microacidometric Test Alice Schauer Weissfeld 1 , Gail D. Sanner 2 , Jack R. Childress 1 , John D. Dyckman 2 , Thomas W. Huber 2 and Robert P. Williams 1 2 Houston City Health Department Laboratory Houston, Texas 77030 1 Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030 ABSTRACT We have shown that it is possible to screen for penicillinase-producing Neisseria gonorrhoeae directly from primary culture plates. Experiments involving the cocultivation of four genera of beta-lactamase-positive (β-lac + ) bacteria and a beta-lactamase-negative (β-lac − ) N. gonorrhoeae on modified Thayer-Martin medium indicated that suppressed or inhibited β-lac + bacteria did not give false-positive results when isolated β-lac − colonies of gonococci were tested. Colonies were assayed for β-lac production by an adaptation of a microacidometric method in which bacteria could be tested before or after the addition of oxidase reagent. Copyright © 1977 American Society for Microbiology CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article doi: 10.1128/​AAC.12.6.703 Antimicrob. Agents Chemother. December 1977 vol. 12 no. 6 703-706 » Abstract PDF Classifications Physiological Effects and Microbial Susceptibility Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of AAC Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Weissfeld, A. S. Articles by Williams, R. P. Search for related content PubMed PubMed citation Articles by Weissfeld, A. S. Articles by Williams, R. P. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 55, issue 12 Alert me to new issues of AAC About AAC Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AAC RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0066-4804 Online ISSN: 1098-6596 Copyright © 2011 by the American Society for Microbiology. For an alternate route to AAC .asm.org, visit: http://intl- AAC .asm.org | More Info» var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-5821458-3"); pageTracker._trackPageview();
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