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Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants (Olea europaea L.)

Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants... Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants ( Olea europaea L.) ▿ † Isabel Pérez-Martínez 1 , ¶ § , Luis Rodríguez-Moreno 1 , ‡ § , Lotte Lambertsen 1 , ‖ , Isabel M. Matas 2 , Jesús Murillo 3 , Stefania Tegli 4 , Antonio J. Jiménez 1 and Cayo Ramos 2 , * 1 Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, Spain 2 Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Área de Genética, Facultad de Ciencias, Universidad de Málaga, Campus Teatinos s/n, E-29010 Málaga, Spain 3 Departamento de Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, E-31006 Pamplona, Spain 4 Dipartimento di Biotecnologie Agrarie, Universitá degli Studi di Firenze, Sez. Patologia Vegetale, Laboratorio di Patologia Vegetale Molecolare, Via della Lastruccia 10, 50019 Sesto Fiorentino, Italy ABSTRACT Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS -to- hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructed a mutant of NCPPB 3335, termed T3, which carries a deletion from the 3′ end of the hrpS gene to the 5′ end of the hrpZ operon. Despite its inability to multiply in olive tissues and to induce tumor formation in woody olive plants, P . savastanoi pv. savastanoi T3 can induce knot formation on young micropropagated olive plants. However, the necrosis and formation of internal open cavities previously reported in knots induced by the wild-type strain were not observed in those induced by P . savastanoi pv. savastanoi T3. Tagging of P . savastanoi pv. savastanoi T3 with green fluorescent protein (GFP) allowed real-time monitoring of its behavior on olive plants. In olive plant tissues, the wild-type strain formed aggregates that colonized the intercellular spaces and internal cavities of the hypertrophic knots, while the mutant T3 strain showed a disorganized distribution within the parenchyma of the knot. Ultrastructural analysis of knot sections revealed the release of extensive outer membrane vesicles from the bacterial cell surface of the P . savastanoi pv. savastanoi T3 mutant, while the wild-type strain exhibited very few vesicles. This phenomenon has not been described before for any other bacterial phytopathogen during host infection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied and Environmental Microbiology American Society For Microbiology

Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants (Olea europaea L.)

Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants (Olea europaea L.)

Applied and Environmental Microbiology , Volume 76 (11): 3611 – Jun 1, 2010

Abstract

Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants ( Olea europaea L.) ▿ † Isabel Pérez-Martínez 1 , ¶ § , Luis Rodríguez-Moreno 1 , ‡ § , Lotte Lambertsen 1 , ‖ , Isabel M. Matas 2 , Jesús Murillo 3 , Stefania Tegli 4 , Antonio J. Jiménez 1 and Cayo Ramos 2 , * 1 Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, Spain 2 Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Área de Genética, Facultad de Ciencias, Universidad de Málaga, Campus Teatinos s/n, E-29010 Málaga, Spain 3 Departamento de Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, E-31006 Pamplona, Spain 4 Dipartimento di Biotecnologie Agrarie, Universitá degli Studi di Firenze, Sez. Patologia Vegetale, Laboratorio di Patologia Vegetale Molecolare, Via della Lastruccia 10, 50019 Sesto Fiorentino, Italy ABSTRACT Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS -to- hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructed a mutant of NCPPB 3335, termed T3, which carries a deletion from the 3′ end of the hrpS gene to the 5′ end of the hrpZ operon. Despite its inability to multiply in olive tissues and to induce tumor formation in woody olive plants, P . savastanoi pv. savastanoi T3 can induce knot formation on young micropropagated olive plants. However, the necrosis and formation of internal open cavities previously reported in knots induced by the wild-type strain were not observed in those induced by P . savastanoi pv. savastanoi T3. Tagging of P . savastanoi pv. savastanoi T3 with green fluorescent protein (GFP) allowed real-time monitoring of its behavior on olive plants. In olive plant tissues, the wild-type strain formed aggregates that colonized the intercellular spaces and internal cavities of the hypertrophic knots, while the mutant T3 strain showed a disorganized distribution within the parenchyma of the knot. Ultrastructural analysis of knot sections revealed the release of extensive outer membrane vesicles from the bacterial cell surface of the P . savastanoi pv. savastanoi T3 mutant, while the wild-type strain exhibited very few vesicles. This phenomenon has not been described before for any other bacterial phytopathogen during host infection.

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References (46)

Publisher
American Society For Microbiology
Copyright
Copyright © 2010 by the American society for Microbiology.
ISSN
0099-2240
eISSN
1098-5336
DOI
10.1128/AEM.00133-10
pmid
20363790
Publisher site
See Article on Publisher Site

Abstract

Fate of a Pseudomonas savastanoi pv. savastanoi Type III Secretion System Mutant in Olive Plants ( Olea europaea L.) ▿ † Isabel Pérez-Martínez 1 , ¶ § , Luis Rodríguez-Moreno 1 , ‡ § , Lotte Lambertsen 1 , ‖ , Isabel M. Matas 2 , Jesús Murillo 3 , Stefania Tegli 4 , Antonio J. Jiménez 1 and Cayo Ramos 2 , * 1 Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, Spain 2 Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Área de Genética, Facultad de Ciencias, Universidad de Málaga, Campus Teatinos s/n, E-29010 Málaga, Spain 3 Departamento de Producción Agraria, Universidad Pública de Navarra, Campus de Arrosadía, E-31006 Pamplona, Spain 4 Dipartimento di Biotecnologie Agrarie, Universitá degli Studi di Firenze, Sez. Patologia Vegetale, Laboratorio di Patologia Vegetale Molecolare, Via della Lastruccia 10, 50019 Sesto Fiorentino, Italy ABSTRACT Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS -to- hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructed a mutant of NCPPB 3335, termed T3, which carries a deletion from the 3′ end of the hrpS gene to the 5′ end of the hrpZ operon. Despite its inability to multiply in olive tissues and to induce tumor formation in woody olive plants, P . savastanoi pv. savastanoi T3 can induce knot formation on young micropropagated olive plants. However, the necrosis and formation of internal open cavities previously reported in knots induced by the wild-type strain were not observed in those induced by P . savastanoi pv. savastanoi T3. Tagging of P . savastanoi pv. savastanoi T3 with green fluorescent protein (GFP) allowed real-time monitoring of its behavior on olive plants. In olive plant tissues, the wild-type strain formed aggregates that colonized the intercellular spaces and internal cavities of the hypertrophic knots, while the mutant T3 strain showed a disorganized distribution within the parenchyma of the knot. Ultrastructural analysis of knot sections revealed the release of extensive outer membrane vesicles from the bacterial cell surface of the P . savastanoi pv. savastanoi T3 mutant, while the wild-type strain exhibited very few vesicles. This phenomenon has not been described before for any other bacterial phytopathogen during host infection.

Journal

Applied and Environmental MicrobiologyAmerican Society For Microbiology

Published: Jun 1, 2010

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