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Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium difficile

Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium... Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium difficile Patrizia Spigaglia and Paola Mastrantonio * Bacteriology and Medical Mycology Laboratory, Istituto Superiore di Sanità, Rome, Italy ABSTRACT Repetitive element sequence-based PCR (rep-PCR) is a typing method that enables the generation of DNA fingerprinting that discriminates bacterial strains. In this study, we evaluated the applicability of rep-PCR in typing Clostridium difficile clinical isolates. The results obtained by rep-PCR were compared with those obtained by pulsed-field gel electrophoresis (PFGE) and PCR ribotyping. A high correspondence between pattern differentiations produced by rep-PCR and PFGE was observed, whereas PCR ribotyping showed a lower level of discriminatory power. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Microbiology American Society For Microbiology

Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium difficile

Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium difficile

Journal of Clinical Microbiology , Volume 41 (6): 2454 – Jun 1, 2003

Abstract

Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium difficile Patrizia Spigaglia and Paola Mastrantonio * Bacteriology and Medical Mycology Laboratory, Istituto Superiore di Sanità, Rome, Italy ABSTRACT Repetitive element sequence-based PCR (rep-PCR) is a typing method that enables the generation of DNA fingerprinting that discriminates bacterial strains. In this study, we evaluated the applicability of rep-PCR in typing Clostridium difficile clinical isolates. The results obtained by rep-PCR were compared with those obtained by pulsed-field gel electrophoresis (PFGE) and PCR ribotyping. A high correspondence between pattern differentiations produced by rep-PCR and PFGE was observed, whereas PCR ribotyping showed a lower level of discriminatory power.

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References (28)

Publisher
American Society For Microbiology
Copyright
Copyright © 2003 by the American society for Microbiology.
ISSN
0095-1137
eISSN
1098-660X
DOI
10.1128/JCM.41.6.2454-2457.2003
Publisher site
See Article on Publisher Site

Abstract

Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium difficile Patrizia Spigaglia and Paola Mastrantonio * Bacteriology and Medical Mycology Laboratory, Istituto Superiore di Sanità, Rome, Italy ABSTRACT Repetitive element sequence-based PCR (rep-PCR) is a typing method that enables the generation of DNA fingerprinting that discriminates bacterial strains. In this study, we evaluated the applicability of rep-PCR in typing Clostridium difficile clinical isolates. The results obtained by rep-PCR were compared with those obtained by pulsed-field gel electrophoresis (PFGE) and PCR ribotyping. A high correspondence between pattern differentiations produced by rep-PCR and PFGE was observed, whereas PCR ribotyping showed a lower level of discriminatory power.

Journal

Journal of Clinical MicrobiologyAmerican Society For Microbiology

Published: Jun 1, 2003

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