EMPLOYMENT OF A DOUBLE SUGAR MEDIUM FOR ROUTINE DIAGNOSIS OF BACILLARY WHITE DIARRHEA, FOWL TYPHOID, AND FOWL CHOLERA
Abstract
Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://jb.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ GEORGE A. CRUICKSHANK Rhode Island Agricultural Experiment Station, Kingston, Rhode Island Received for publication June 7, 127 The ordinary procedure in diagnosing the common poultry diseases consists in making cultures on agar slants and, after growth has appeared, transferring the culture to four fermentation tubes containing respectively lactose, glucose, sucrose, and either maltose, dextrin, or dulcitol. The fermentation of glucose alone, either with or without the production of gas, indicates the presence of Salmonella pullorum, the cause of bacillary white diarrhea. If both glucose and maltose (dextrin or dulcitol may be used in place of maltose) are fermented, but neither lactose nor sucrose, the organism is Salmonella gallinarum, the cause of fowl typhoid. Acid in glucose and sucrose, but not in lactose or in maltose, shows the organism to be that of fowl cholera (Pasteurella avicida)'. Hadley and his co-workers (1918) showed that Salmonella pullorum and Salmonella gallinarum produce approximately the same amount of acid in glucose. Most cultures of S. pullorum produce gas in glucose. Pasteurella avicida