Effectiveness of an Integrated Real-Time PCR Method for Detection of the Mycobacterium tuberculosis Complex in Smear-Negative Extrapulmonary Samples in an Area of Low Tuberculosis Prevalence Raquel Moure a , Rogelio Martín a , b and Fernando Alcaide a , b a Department of Microbiology, IDIBELL-Bellvitge Hospital b Department of Pathology and Experimental Therapeutics, University of Barcelona, Feixa Llarga, Hospitalet de Llobregat (Barcelona), Spain ABSTRACT Early extrapulmonary tuberculosis (EPTB) diagnosis is particularly difficult. Among 108 smear-negative extrapulmonary samples showing a positive culture for Mycobacterium tuberculosis complex (43 body fluids and 65 nonliquid specimens), 63 (58.3%) were positive with the Xpert MTB/RIF assay (GX). GX sensitivity was quite low for samples from sterile locations (especially for pleural fluids: 26.9%) but high for some nonliquid samples, like abscess aspirates (76.5%). In summary, GX may be a useful tool to be considered for EPTB diagnosis.
Effectiveness of an Integrated Real-Time PCR Method for Detection of the Mycobacterium tuberculosis Complex in Smear-Negative Extrapulmonary Samples in an Area of Low Tuberculosis Prevalence
Abstract
Effectiveness of an Integrated Real-Time PCR Method for Detection of the Mycobacterium tuberculosis Complex in Smear-Negative Extrapulmonary Samples in an Area of Low Tuberculosis Prevalence Raquel Moure a , Rogelio Martín a , b and Fernando Alcaide a , b a Department of Microbiology, IDIBELL-Bellvitge Hospital b Department of Pathology and Experimental Therapeutics, University of Barcelona, Feixa Llarga, Hospitalet de Llobregat (Barcelona), Spain ABSTRACT Early extrapulmonary tuberculosis (EPTB) diagnosis is particularly difficult. Among 108 smear-negative extrapulmonary samples showing a positive culture for Mycobacterium tuberculosis complex (43 body fluids and 65 nonliquid specimens), 63 (58.3%) were positive with the Xpert MTB/RIF assay (GX). GX sensitivity was quite low for samples from sterile locations (especially for pleural fluids: 26.9%) but high for some nonliquid samples, like abscess aspirates (76.5%). In summary, GX may be a useful tool to be considered for EPTB diagnosis.Preview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Effectiveness of an Integrated Real-Time PCR Method for Detection of the Mycobacterium tuberculosis Complex in Smear-Negative Extrapulmonary Samples in an Area of Low Tuberculosis Prevalence
Moure, Raquel; Martín, Rogelio; Alcaide, Fernando
Follow Journal of Clinical Microbiology
, Volume 50 (2): 513
American Society For Microbiology – Feb 1, 2012
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