Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Discrimination of Human Pathogenic Subspecies of Francisella tularensis by Using Restriction Fragment Length Polymorphism

Discrimination of Human Pathogenic Subspecies of Francisella tularensis by Using Restriction... Discrimination of Human Pathogenic Subspecies of Francisella tularensis by Using Restriction Fragment Length Polymorphism Rebecca Thomas 1 , * , Anders Johansson 2 , 3 , 4 , Brendan Neeson 1 , Karen Isherwood 1 , Anders Sjöstedt 2 , Jill Ellis 1 and Richard W. Titball 1 , 5 1 Defence Science and Technology Laboratory, CBS Porton Down, Salisbury, Wiltshire SP4 0JQ 5 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom 2 Department of Clinical Microbiology, Clinical Bacteriology 3 Department of Clinical Microbiology, Infectious Diseases, Umeå University, SE-901 85 Umeå 4 NBC-Analysis Swedish Defence Research Agency, SE-901 82 Umeå, Sweden ABSTRACT We describe the use of two insertion sequence elements (ISFtu1 and ISFtu2) in Francisella tularensis to type strains by restriction fragment length polymorphism (RFLP). The RFLP profiles of 17 epidemiologically unrelated isolates were determined and compared. Our results showed that RFLP profiles can be used to assign F. tularensis strains into five main groups corresponding to strains of F. tularensis subsp. tularensis , F. tularensis strain ATCC 6223, strains of F. tularensis subsp. holarctica , strains of F. tularensis subsp. holarctica from Japan, and F. tularensis subsp. mediaasiatica . The results confirm the genetic identities of these subspecies and also support the suggestion that strains of F. tularensis subsp. holarctica from Japan should be considered members of a separate biovar. These findings should support future studies to determine the genetic differences between strains of F. tularensis at the whole-genome level. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Microbiology American Society For Microbiology

Discrimination of Human Pathogenic Subspecies of Francisella tularensis by Using Restriction Fragment Length Polymorphism

Discrimination of Human Pathogenic Subspecies of Francisella tularensis by Using Restriction Fragment Length Polymorphism

Journal of Clinical Microbiology , Volume 41 (1): 50 – Jan 1, 2003

Abstract

Discrimination of Human Pathogenic Subspecies of Francisella tularensis by Using Restriction Fragment Length Polymorphism Rebecca Thomas 1 , * , Anders Johansson 2 , 3 , 4 , Brendan Neeson 1 , Karen Isherwood 1 , Anders Sjöstedt 2 , Jill Ellis 1 and Richard W. Titball 1 , 5 1 Defence Science and Technology Laboratory, CBS Porton Down, Salisbury, Wiltshire SP4 0JQ 5 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom 2 Department of Clinical Microbiology, Clinical Bacteriology 3 Department of Clinical Microbiology, Infectious Diseases, Umeå University, SE-901 85 Umeå 4 NBC-Analysis Swedish Defence Research Agency, SE-901 82 Umeå, Sweden ABSTRACT We describe the use of two insertion sequence elements (ISFtu1 and ISFtu2) in Francisella tularensis to type strains by restriction fragment length polymorphism (RFLP). The RFLP profiles of 17 epidemiologically unrelated isolates were determined and compared. Our results showed that RFLP profiles can be used to assign F. tularensis strains into five main groups corresponding to strains of F. tularensis subsp. tularensis , F. tularensis strain ATCC 6223, strains of F. tularensis subsp. holarctica , strains of F. tularensis subsp. holarctica from Japan, and F. tularensis subsp. mediaasiatica . The results confirm the genetic identities of these subspecies and also support the suggestion that strains of F. tularensis subsp. holarctica from Japan should be considered members of a separate biovar. These findings should support future studies to determine the genetic differences between strains of F. tularensis at the whole-genome level.

Loading next page...
 
/lp/american-society-for-microbiology/discrimination-of-human-pathogenic-subspecies-of-francisella-h0YZa09pYQ

References (36)

Publisher
American Society For Microbiology
Copyright
Copyright © 2003 by the American society for Microbiology.
ISSN
0095-1137
eISSN
1098-660X
DOI
10.1128/JCM.41.1.50-57.2003
Publisher site
See Article on Publisher Site

Abstract

Discrimination of Human Pathogenic Subspecies of Francisella tularensis by Using Restriction Fragment Length Polymorphism Rebecca Thomas 1 , * , Anders Johansson 2 , 3 , 4 , Brendan Neeson 1 , Karen Isherwood 1 , Anders Sjöstedt 2 , Jill Ellis 1 and Richard W. Titball 1 , 5 1 Defence Science and Technology Laboratory, CBS Porton Down, Salisbury, Wiltshire SP4 0JQ 5 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom 2 Department of Clinical Microbiology, Clinical Bacteriology 3 Department of Clinical Microbiology, Infectious Diseases, Umeå University, SE-901 85 Umeå 4 NBC-Analysis Swedish Defence Research Agency, SE-901 82 Umeå, Sweden ABSTRACT We describe the use of two insertion sequence elements (ISFtu1 and ISFtu2) in Francisella tularensis to type strains by restriction fragment length polymorphism (RFLP). The RFLP profiles of 17 epidemiologically unrelated isolates were determined and compared. Our results showed that RFLP profiles can be used to assign F. tularensis strains into five main groups corresponding to strains of F. tularensis subsp. tularensis , F. tularensis strain ATCC 6223, strains of F. tularensis subsp. holarctica , strains of F. tularensis subsp. holarctica from Japan, and F. tularensis subsp. mediaasiatica . The results confirm the genetic identities of these subspecies and also support the suggestion that strains of F. tularensis subsp. holarctica from Japan should be considered members of a separate biovar. These findings should support future studies to determine the genetic differences between strains of F. tularensis at the whole-genome level.

Journal

Journal of Clinical MicrobiologyAmerican Society For Microbiology

Published: Jan 1, 2003

There are no references for this article.