Discovering Novel Bile Protection Systems in Bifidobacterium breve UCC2003 through Functional Genomics Lorena Ruiz a , b , Aldert Zomer b , * , Mary O'Connell-Motherway b , Douwe van Sinderen b and Abelardo Margolles a a Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias-CSIC, Villaviciosa, Asturias, Spain b Department of Microbiology and Alimentary Pharmabiotic Centre, University College of Cork, Cork, Ireland ABSTRACT Tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. A transcriptomic approach was employed to study the response of Bifidobacterium breve UCC2003 to bile, allowing the identification of a number of bile-induced genes with a range of predicted functions. The potential roles of a selection of these bile-inducible genes in bile protection were analyzed following heterologous expression in Lactococcus lactis . Genes encoding three transport systems belonging to the major facilitator superfamily (MFS), Bbr_0838, Bbr_0832, and Bbr_1756, and three ABC-type transporters, Bbr_0406-0407, Bbr_1804-1805, and Bbr_1826-1827, were thus investigated and shown to provide enhanced resistance and survival to bile exposure. This work significantly improves our understanding as to how bifidobacteria respond to and survive bile exposure.
Discovering Novel Bile Protection Systems in Bifidobacterium breve UCC2003 through Functional Genomics
Abstract
Discovering Novel Bile Protection Systems in Bifidobacterium breve UCC2003 through Functional Genomics Lorena Ruiz a , b , Aldert Zomer b , * , Mary O'Connell-Motherway b , Douwe van Sinderen b and Abelardo Margolles a a Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias-CSIC, Villaviciosa, Asturias, Spain b Department of Microbiology and Alimentary Pharmabiotic Centre, University College of Cork, Cork, Ireland ABSTRACT Tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. A transcriptomic approach was employed to study the response of Bifidobacterium breve UCC2003 to bile, allowing the identification of a number of bile-induced genes with a range of predicted functions. The potential roles of a selection of these bile-inducible genes in bile protection were analyzed following heterologous expression in Lactococcus lactis . Genes encoding three transport systems belonging to the major facilitator superfamily (MFS), Bbr_0838, Bbr_0832, and Bbr_1756, and three ABC-type transporters, Bbr_0406-0407, Bbr_1804-1805, and Bbr_1826-1827, were thus investigated and shown to provide enhanced resistance and survival to bile exposure. This work significantly improves our understanding as to how bifidobacteria respond to and survive bile exposure.Preview Only. This article cannot be rented because we do not currently have permission from the publisher.
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Discovering Novel Bile Protection Systems in Bifidobacterium breve UCC2003 through Functional Genomics
Ruiz, Lorena; Zomer, Aldert; O'Connell-Motherway, Mary; van Sinderen, Douwe; Margolles, Abelardo
Follow Applied and Environmental Microbiology
, Volume 78 (4): 1123
American Society For Microbiology – Feb 15, 2012
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