Abstract

Dioxygenation of the Biphenyl Dioxygenation Product Heike Overwin a , Myriam González b , Valentina Méndez b , Michael Seeger b , Victor Wray c and Bernd Hofer a a Department of Chemical Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany b Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Departamento de Química, Universidad Técnica Federico Santa María, Valparaíso, Chile c Department of Molecular Structural Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany ABSTRACT Two biphenyl dioxygenases (BphAs) were shown to catalyze dioxygenation of biphenyldienediol in the nonoxidized ring to form the respective symmetrical biphenyl-bis-dienediol. This novel metabolite served as a growth substrate for both BphA source strains. Its catabolism through the upper bph pathway of Burkholderia xenovorans LB400 was analyzed.