Comparison of the Vitek Immunodiagnostic Assay System with an indirect immunoassay (Toxostat Test Kit) for detection of immunoglobulin G antibodies to Toxoplasma gondii in clinical specimens.
Abstract
Comparison of the Vitek Immunodiagnostic Assay System with an indirect immunoassay (Toxostat Test Kit) for detection of immunoglobulin G antibodies to Toxoplasma gondii in clinical specimens. R L Sandin , C C Knapp , G S Hall , J A Washington and I Rutherford Department of Microbiology, Cleveland Clinic Foundation, Ohio 44195. ABSTRACT The value and convenience of testing for specific anti-Toxoplasma gondii antibodies have led to the development of various antibody detection methods, such as the enzyme immunoassay. Two enzyme immunoassays, the Vitek Immuno-Diagnostic Assay System (VIDAS; Vitek Systems, Hazelwood, Mo.) and the Toxostat Test Kit (TST; Whittaker Bioproducts, Walkersville, Md.), were compared for their ability to detect T. gondii immunoglobulin G antibodies in fresh human sera. Specimens were tested according to the instructions of each manufacturer. Of 304 serum specimens tested, the results for 282 (93%) agreed in both assays (77 were positive and 205 were negative). The results for the remaining 22 (7%) specimens were discrepant; 20 of the 22 specimens tested low positive or equivocal by Toxostat and negative by VIDAS. Upon retesting, 8 of these 22 specimens were in concordance, increasing the level of agreement to 95.3%. Intra-assay reproducibility was tested with four to nine replicates of each of seven samples (four positive, one negative, and two equivocal) by both tests and six replicates of one standard by VIDAS. Coefficients of variation (CVs) for VIDAS were 6, 8, 10, 15, 18, 19, and 23% for the samples and 14% for the standard. For Toxostat the CVs were 2, 8, 10, 10, 13, and 16%. In general, CVs for VIDAS were slightly larger than the CVs for Toxostat. Day-to-day variability over 3 days was tested by VIDAS for only six samples (three positive, two negative, and one equivocal) and four standards; CVs ranged from 2 to 15% for the samples and from 6 to 13% for the standards. In conclusions, VIDAS is a rapid, convenient, non-labor-intensive, and easily performed test for the detection of immunoglobulin G antibodies to T. gondii in serum specimens. CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article J. Clin. Microbiol. December 1991 vol. 29 no. 12 2763-2767 » Abstract PDF Classifications Research Article Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of JCM Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by Sandin, R. L. Articles by Rutherford, I. Search for related content PubMed PubMed citation Articles by Sandin, R. L. Articles by Rutherford, I. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 49, issue 12 Alert me to new issues of JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2011 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: http://intl- JCM .asm.org | More Info» var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www."); document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E")); var pageTracker = _gat._getTracker("UA-5821458-10"); pageTracker._trackPageview();