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CD4-Independent Infection of Two CD4−/CCR5−/CXCR4+ Pre-T-Cell Lines by Human and Simian Immunodeficiency Viruses

CD4-Independent Infection of Two CD4−/CCR5−/CXCR4+ Pre-T-Cell Lines by Human and Simian... CD4-Independent Infection of Two CD4 − /CCR5 − /CXCR4 + Pre-T-Cell Lines by Human and Simian Immunodeficiency Viruses Alessandra Borsetti 1 , * , Cristina Parolin 2 , Barbara Ridolfi 1 , Leonardo Sernicola 1 , Andrea Geraci 1 , Barbara Ensoli 1 , and Fausto Titti 1 Laboratory of Virology, Istituto Superiore di Sanità, Rome, 1 and Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, 2 Italy ABSTRACT The infection of CD4-negative cells by variants of tissue culture-adapted human immunodeficiency virus type 1 (HIV-1) or HIV-2 strains has been shown to be mediated by the CXCR4 coreceptor. Here we show that two in vitro-established CD4 − /CCR5 − /CXCR4 + human pre-T-cell lines (A3 and A5) can be productively infected by wild-type laboratory-adapted T-cell-tropic HIV-1 and HIV-2 strains in a CD4-independent, CXCR4-dependent fashion. Despite the absence of CCR5 expression, A3 and A5 cells were susceptible to infection by the simian immunodeficiency viruses SIVmac239 and SIVmac316. Thus, at least in A3 and A5 cells, one or more of the chemokine receptors can efficiently support the entry of HIV and SIV isolates in the absence of CD4. These findings suggest that to infect cells of different compartments, HIV and SIV could have evolved in vivo to bypass CD4 and to interact directly with an alternative receptor. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virology American Society For Microbiology

CD4-Independent Infection of Two CD4−/CCR5−/CXCR4+ Pre-T-Cell Lines by Human and Simian Immunodeficiency Viruses

CD4-Independent Infection of Two CD4−/CCR5−/CXCR4+ Pre-T-Cell Lines by Human and Simian Immunodeficiency Viruses

Journal of Virology , Volume 74 (14): 6689 – Jul 15, 2000

Abstract

CD4-Independent Infection of Two CD4 − /CCR5 − /CXCR4 + Pre-T-Cell Lines by Human and Simian Immunodeficiency Viruses Alessandra Borsetti 1 , * , Cristina Parolin 2 , Barbara Ridolfi 1 , Leonardo Sernicola 1 , Andrea Geraci 1 , Barbara Ensoli 1 , and Fausto Titti 1 Laboratory of Virology, Istituto Superiore di Sanità, Rome, 1 and Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, 2 Italy ABSTRACT The infection of CD4-negative cells by variants of tissue culture-adapted human immunodeficiency virus type 1 (HIV-1) or HIV-2 strains has been shown to be mediated by the CXCR4 coreceptor. Here we show that two in vitro-established CD4 − /CCR5 − /CXCR4 + human pre-T-cell lines (A3 and A5) can be productively infected by wild-type laboratory-adapted T-cell-tropic HIV-1 and HIV-2 strains in a CD4-independent, CXCR4-dependent fashion. Despite the absence of CCR5 expression, A3 and A5 cells were susceptible to infection by the simian immunodeficiency viruses SIVmac239 and SIVmac316. Thus, at least in A3 and A5 cells, one or more of the chemokine receptors can efficiently support the entry of HIV and SIV isolates in the absence of CD4. These findings suggest that to infect cells of different compartments, HIV and SIV could have evolved in vivo to bypass CD4 and to interact directly with an alternative receptor.

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References (38)

Publisher
American Society For Microbiology
Copyright
Copyright © 2000 by the American society for Microbiology.
ISSN
0022-538X
eISSN
1098-5514
DOI
10.1128/JVI.74.14.6689-6694.2000
Publisher site
See Article on Publisher Site

Abstract

CD4-Independent Infection of Two CD4 − /CCR5 − /CXCR4 + Pre-T-Cell Lines by Human and Simian Immunodeficiency Viruses Alessandra Borsetti 1 , * , Cristina Parolin 2 , Barbara Ridolfi 1 , Leonardo Sernicola 1 , Andrea Geraci 1 , Barbara Ensoli 1 , and Fausto Titti 1 Laboratory of Virology, Istituto Superiore di Sanità, Rome, 1 and Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, 2 Italy ABSTRACT The infection of CD4-negative cells by variants of tissue culture-adapted human immunodeficiency virus type 1 (HIV-1) or HIV-2 strains has been shown to be mediated by the CXCR4 coreceptor. Here we show that two in vitro-established CD4 − /CCR5 − /CXCR4 + human pre-T-cell lines (A3 and A5) can be productively infected by wild-type laboratory-adapted T-cell-tropic HIV-1 and HIV-2 strains in a CD4-independent, CXCR4-dependent fashion. Despite the absence of CCR5 expression, A3 and A5 cells were susceptible to infection by the simian immunodeficiency viruses SIVmac239 and SIVmac316. Thus, at least in A3 and A5 cells, one or more of the chemokine receptors can efficiently support the entry of HIV and SIV isolates in the absence of CD4. These findings suggest that to infect cells of different compartments, HIV and SIV could have evolved in vivo to bypass CD4 and to interact directly with an alternative receptor.

Journal

Journal of VirologyAmerican Society For Microbiology

Published: Jul 15, 2000

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