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Ballistic Disruption of Penicillium chrysogenum Cells

Ballistic Disruption of Penicillium chrysogenum Cells Ballistic Disruption of Penicillium chrysogenum Cells Pat B. Hamilton 1 and S. G. Knight Department of Bacteriology, University of Wisconsin, Madison, Wisconsin ABSTRACT The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in a 1:2:3 (w/w) ratio gave above 95% breakage in 15 min with Penicillium chrysogenum cells and in 4 min with Rhizopus nigricans . Some of the factors influencing breakage are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied and Environmental Microbiology American Society For Microbiology

Ballistic Disruption of Penicillium chrysogenum Cells

Ballistic Disruption of Penicillium chrysogenum Cells

Applied and Environmental Microbiology , Volume 10 (6): 577 – Nov 1, 1962

Abstract

Ballistic Disruption of Penicillium chrysogenum Cells Pat B. Hamilton 1 and S. G. Knight Department of Bacteriology, University of Wisconsin, Madison, Wisconsin ABSTRACT The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in a 1:2:3 (w/w) ratio gave above 95% breakage in 15 min with Penicillium chrysogenum cells and in 4 min with Rhizopus nigricans . Some of the factors influencing breakage are discussed.

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Publisher
American Society For Microbiology
Copyright
Copyright © 1962 by the American society for Microbiology.
ISSN
0099-2240
eISSN
1098-5336
Publisher site
See Article on Publisher Site

Abstract

Ballistic Disruption of Penicillium chrysogenum Cells Pat B. Hamilton 1 and S. G. Knight Department of Bacteriology, University of Wisconsin, Madison, Wisconsin ABSTRACT The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in a 1:2:3 (w/w) ratio gave above 95% breakage in 15 min with Penicillium chrysogenum cells and in 4 min with Rhizopus nigricans . Some of the factors influencing breakage are discussed.

Journal

Applied and Environmental MicrobiologyAmerican Society For Microbiology

Published: Nov 1, 1962

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