Application of the peroxidase-antiperoxidase immunoassay to the identification of Salmonellae from pure culture and animal tissue.

Application of the peroxidase-antiperoxidase immunoassay to the identification of Salmonellae from pure culture and animal tissue. C M McRill , T T Kramer and R W Griffith ABSTRACT The peroxidase-antiperoxidase immunoassay was developed by using selected Salmonella serotypes to evaluate its potential for use in diagnostic bacteriology. S. choleraesuis var, kunzendorf, S. dublin, and S. typhimurium were the test organisms. Strong specific staining with corresponding antiserum was achieved with smears of each Salmonella serotype on microscope slides from formalinized cell suspensions, live broth cultures of clinical isolates, and tissue suspensions from the livers and spleens of experimentally infected mice. In addition, S. choleraesuis var. kunzendorf was detected in Formalin-fixed and fresh frozen tissues from experimentally infected pigs. The results of this study indicate that the peroxidase-antiperoxidase assay is well-suited for the rapid identification of Salmonella from pure cultures and that the technique can be useful in research for the detection of this pathogen in histological sections. CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? « Previous | Next Article » Table of Contents This Article J. Clin. Microbiol. August 1984 vol. 20 no. 2 281-284 » Abstract PDF Classifications Research Article Services Email this article to a colleague Similar articles in ASM journals Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of JCM Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Load citing article information Citing articles via Web of Science Citing articles via Google Scholar Google Scholar Articles by McRill, C. M. Articles by Griffith, R. W. Search for related content PubMed PubMed citation Articles by McRill, C. M. Articles by Griffith, R. W. Related Content Load related web page information Social Bookmarking CiteULike Connotea Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter What's this? current issue December 2011, volume 49, issue 12 Alert me to new issues of JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • [email protected] Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2011 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: http://intl- JCM .asm.org | More Info»