Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

An Autonomously Replicating Transforming Vector forSulfolobus solfataricus

An Autonomously Replicating Transforming Vector forSulfolobus solfataricus An Autonomously Replicating Transforming Vector for Sulfolobus solfataricus Raffaele Cannio 1 , Patrizia Contursi 1 , Mosè Rossi 2 and Simonetta Bartolucci 1 , * Dipartimento di Chimica Organica e Biologica, Università di Napoli, 16-80134 Naples, 1 and Istituto di Biochimica delle Proteine ed Enzimologia del CNR, 10-80124 Naples, 2 Italy ABSTRACT A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable mutant of the hph (hygromycin phosphotransferase) gene as the transformation marker. The vector suffered no rearrangement and/or chromosome integration, and its copy number in Sulfolobus was increased by exposure of the cells to mitomycin C. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Bacteriology American Society For Microbiology

An Autonomously Replicating Transforming Vector forSulfolobus solfataricus

An Autonomously Replicating Transforming Vector forSulfolobus solfataricus

Journal of Bacteriology , Volume 180 (12): 3237 – Jun 15, 1998

Abstract

An Autonomously Replicating Transforming Vector for Sulfolobus solfataricus Raffaele Cannio 1 , Patrizia Contursi 1 , Mosè Rossi 2 and Simonetta Bartolucci 1 , * Dipartimento di Chimica Organica e Biologica, Università di Napoli, 16-80134 Naples, 1 and Istituto di Biochimica delle Proteine ed Enzimologia del CNR, 10-80124 Naples, 2 Italy ABSTRACT A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable mutant of the hph (hygromycin phosphotransferase) gene as the transformation marker. The vector suffered no rearrangement and/or chromosome integration, and its copy number in Sulfolobus was increased by exposure of the cells to mitomycin C.

Loading next page...
 
/lp/american-society-for-microbiology/an-autonomously-replicating-transforming-vector-forsulfolobus-n8va44L10A

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
American Society For Microbiology
Copyright
Copyright © 1998 by the American society for Microbiology.
ISSN
0021-9193
eISSN
1098-5530
Publisher site
See Article on Publisher Site

Abstract

An Autonomously Replicating Transforming Vector for Sulfolobus solfataricus Raffaele Cannio 1 , Patrizia Contursi 1 , Mosè Rossi 2 and Simonetta Bartolucci 1 , * Dipartimento di Chimica Organica e Biologica, Università di Napoli, 16-80134 Naples, 1 and Istituto di Biochimica delle Proteine ed Enzimologia del CNR, 10-80124 Naples, 2 Italy ABSTRACT A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable mutant of the hph (hygromycin phosphotransferase) gene as the transformation marker. The vector suffered no rearrangement and/or chromosome integration, and its copy number in Sulfolobus was increased by exposure of the cells to mitomycin C.

Journal

Journal of BacteriologyAmerican Society For Microbiology

Published: Jun 15, 1998

There are no references for this article.