An Autonomously Replicating Transforming Vector forSulfolobus solfataricus
Abstract
An Autonomously Replicating Transforming Vector for Sulfolobus solfataricus Raffaele Cannio 1 , Patrizia Contursi 1 , Mosè Rossi 2 and Simonetta Bartolucci 1 , * Dipartimento di Chimica Organica e Biologica, Università di Napoli, 16-80134 Naples, 1 and Istituto di Biochimica delle Proteine ed Enzimologia del CNR, 10-80124 Naples, 2 Italy ABSTRACT A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable mutant of the hph (hygromycin phosphotransferase) gene as the transformation marker. The vector suffered no rearrangement and/or chromosome integration, and its copy number in Sulfolobus was increased by exposure of the cells to mitomycin C.