Actinobacterial Community Structure in Soils Receiving Long-Term Organic and Inorganic Amendments

Actinobacterial Community Structure in Soils Receiving Long-Term Organic and Inorganic Amendments ▿ † Zhe Piao 1 , 3 , Linzhang Yang 1 , * , Liping Zhao 2 and Shixue Yin 1 , 3 1 Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, People's Republic of China 2 Laboratory of Molecular Microbial Ecology and Ecogenomics, College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, China 3 College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225009, Jiangsu Province, People's Republic of China ABSTRACT The impact of long-term organic and inorganic amendments on the actinobacterial community in soils was studied. Denaturing gradient gel electrophoresis patterns based on the V3 region of 16S rRNA suggested that there was no significant difference between the communities occurring in the different amendments. However, analysis of the clone libraries of the actinobacterial communities by the use of multiple statistical approaches showed that these communities were significantly different from each other. Results showed that long-term organic and inorganic soil amendments did not significantly alter the overall phylogenetic diversity of the actinobacterial communities but did significantly change the community structure.