A Method for the Rapid Cultivation of Desulfovibrio aestuarii on Filter Membranes
Abstract
Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://aem.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ A Method for the Rapid Cultivation of Desulfovibrio aestuarii Filter Membranes1 N. TsuNEISHI2 AND on A. GOETZ California Institute of Technology, Pasadena, California Received for publication July 8, 1957 The cultivation of sulfate-reducing bacteria on agar plates is a somewhat involved and time-consuming process. This is partly due to the requirement for fairly strict anaerobic conditions but mainly because of the length of the incubation period which may vary from 6 days to weeks with the resulting delay of the required information. The work reported herein was undertaken in an attempt to apply the advantageous features of the membrane filter method to the detection of anaerobic organisms. As originally developed, the membrane filter (Goetz and Tsuneishi, 1951; Clark et al., 1951) permits the recovery of small numbers of organisms from large volumes of water and the rapid cultivation of many aerobic bacteria. The ultimate purpose was to develop a method which would not require more complex manipulation than the membrane procedure according to Standard Methods for the Examination