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A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid Bacteria

A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid... A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid Bacteria † L. C. Mc Donald , R. F. Mc Feeters * , M. A. Daeschel and H. P. Fleming Food Fermentation Laboratory, Agricultural Research Service, U.S. Department of Agriculture, and Department of Food Science, North Carolina Agricultural Research Service, North Carolina State University, Raleigh, North Carolina 27695-7624 ABSTRACT A medium was developed for the differential enumeration of homofermentative and heterofermentative lactic acid bacteria. Essential components of the medium included fructose (14 mM), KH 2 PO 4 (18 mM), bromcresol green (as a pH indicator), and other nutrients to support growth. In agar medium, homofermentative colonies were blue to green, while heterofermentative colonies remained white. A total of 21 Lactobacillus, Pediococcus, Leuconostoc, and Streptococcus species were correctly classified with the medium. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied and Environmental Microbiology American Society For Microbiology

A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid Bacteria

A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid Bacteria

Applied and Environmental Microbiology , Volume 53 (6): 1382 – Jun 1, 1987

Abstract

A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid Bacteria † L. C. Mc Donald , R. F. Mc Feeters * , M. A. Daeschel and H. P. Fleming Food Fermentation Laboratory, Agricultural Research Service, U.S. Department of Agriculture, and Department of Food Science, North Carolina Agricultural Research Service, North Carolina State University, Raleigh, North Carolina 27695-7624 ABSTRACT A medium was developed for the differential enumeration of homofermentative and heterofermentative lactic acid bacteria. Essential components of the medium included fructose (14 mM), KH 2 PO 4 (18 mM), bromcresol green (as a pH indicator), and other nutrients to support growth. In agar medium, homofermentative colonies were blue to green, while heterofermentative colonies remained white. A total of 21 Lactobacillus, Pediococcus, Leuconostoc, and Streptococcus species were correctly classified with the medium.

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Publisher
American Society For Microbiology
Copyright
Copyright © 1987 by the American society for Microbiology.
ISSN
0099-2240
eISSN
1098-5336
Publisher site
See Article on Publisher Site

Abstract

A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid Bacteria † L. C. Mc Donald , R. F. Mc Feeters * , M. A. Daeschel and H. P. Fleming Food Fermentation Laboratory, Agricultural Research Service, U.S. Department of Agriculture, and Department of Food Science, North Carolina Agricultural Research Service, North Carolina State University, Raleigh, North Carolina 27695-7624 ABSTRACT A medium was developed for the differential enumeration of homofermentative and heterofermentative lactic acid bacteria. Essential components of the medium included fructose (14 mM), KH 2 PO 4 (18 mM), bromcresol green (as a pH indicator), and other nutrients to support growth. In agar medium, homofermentative colonies were blue to green, while heterofermentative colonies remained white. A total of 21 Lactobacillus, Pediococcus, Leuconostoc, and Streptococcus species were correctly classified with the medium.

Journal

Applied and Environmental MicrobiologyAmerican Society For Microbiology

Published: Jun 1, 1987

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