Abstract

Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this article), more» Information about commercial reprint orders: http://jb.asm.org/site/misc/reprints.xhtml To subscribe to to another ASM Journal go to: http://journals.asm.org/site/subscriptions/ Bureau of Bacteriology, Maryland State Department of Health, Baltimore, Maryland Received for publication July 3, 1935 The routine use of a Modified Eijkman method in the examination of oysters, crabmeat, and other substances has been discussed by the authors (1935). Only three sewage samples were reported, a number obviously insufficient from which to draw any conclusion, especially since these observations were made before certain improvements in the Eijkman method had been applied. Therefore, it was thought desirable to study the method further with samples of raw sewage, effluent and sludge in order to evaluate more accurately the authors' modification of the Eijkman test for this purpose. Brown and Skinner (1930) have criticized the value of the test based on pure cultures, alone. In the present investigation, the dilution method, using triplicate inoculations for each dilution, has been used to compare the Eijkman test with other tests used for the examination of water in different parts of America and with the Standard lactose broth method. PROCEDURE High dilutions of