Activated sludge from sewage treatment plant was tested for their suitability as inoculum source for the fermentation H 2 production. A series of batch experiments were conducted to study the effects of various sludge pretreatments (domestication, boiling, acid, and alkali) on the fermentative H2 production from apple residue. The sludge domestication process markedly shortens the H2-producing lag time, and H2 gas was produced only 5 min after mixing the sludge and substrate. The boiling pretreatment of the sludge is better than the acid and alkali pretreatments in enhancing hydrogen yield from apple residue. The best domestication and boiling time for the sludge were 6 h and 30 min, respectively. A maximum cumulative H 2 yield of 87.33 ml·g -1 -TS with an average H 2 production rate of 14.56 ml·g -1 -TS·h -1 was obtained at the fermentation temperature of 37 °C, initial p H 7.0, and substrate concentrate of 20 g·l -1 .
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