Interstitial Chromosomal Deletion within 4q11-q13 in a Human Hepatoma Cell Line
AbstractSouthern blot analysis revealed the presence of an aberrant albumin gene as well as a normal one in a human hepatoma cell line, HuH-7. A genomic sequence carrying this altered gene was isolated and characterized. This clone contains a 3-kbp 3' segment of the albumin gene linked to a non-albumin sequence at intron 11. The non-albumin sequence is assigned to chromosome 4q12–q13 by in situ hybridization. This indicates an interstitial deletion of a chromosomal segment within 4q11–q13 because the albumin gene is mapped there. The truncated albumin gene is detected in an early passage of HuH-7 cells and has been maintained stably in cell culture. 1 This work was supported by the National Cancer Institute of Canada, the Medical Research Council of Canada, and Japan Immuno Research Laboratory, Inc. 2 Present address: Department of Dermatology, University of Tokushima, Tokushima 770, Japan. 3 Present address: Department of Biochemical Engineering and Science. Kyushu Institute of Technology, lizuka, Fukuoka 820, Japan. 4 To whom requests for reprints should be addressed, at Department of Medical Biochemistry, University of Calgary, Calgary, Alberta T2N 4N1, Canada.