An Immunoelectron Microscopy Study of Soehner-Dmochowski Murine Sarcoma Virus following Passage in Rats and Hamsters
Abstract
Immunoelectron microscopy techniques, using immunoferitin and immunoperoxidase, were applied to the study of viral envelope antigens following passage of the Soehner-Dmochowski isolate of the murine sarcoma virus in rats and hamsters. Results of this study have demonstrated that the envelope antigens of Soehner-Dmochowski murine sarcoma virus recovered from rat bone tumor cells were distinct from those of Soehner-Dmochowski murine sarcoma virus recovered from the hamster bone tumor cells. The usefulness of these immunoelectron microscopy techniques is illustrated. 1 This study was supported in part by USPHS Contract NO1-CP-33304 within the Virus Cancer Program of the National Cancer Institute, NIH, Bethesda, Md.; by Grants CA-05831 and RR-05511 from the National Cancer Institute, NIH, USPHS; and by a grant from the Leukemia Society of America, Inc. 2 To whom requests for reprints should be addressed.