Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Purification of Two Basic 1, 3-b-Glucanase Isoforms from Cyamopsis tetragonoloba (L.)Taub. Induced to Resist Virus Infections

Purification of Two Basic 1, 3-b-Glucanase Isoforms from Cyamopsis tetragonoloba (L.)Taub.... 1,3-b-glucanases (EC 3.2.1.39) are enzymes that degrade polysaccharidic substratesand have antimicrobial activity against fungi and bacteria. They have also been implicated in systemic acquired resistance (SAR) indirectly through release of endogenous elicitor molecules. SAR against viruses can be induced in plants by a few substances, including proteins isolated from some non-host plants. In the present study, CAP-34, a 34 kDa basic protein isolated from Clerodendrumaculeatum L., was used to induce systemic resistance against sunnhemp rosette virusin Cyamopsis tetragonoloba (L.) Taub. 1,3-b-glucanase activity increased rapidlyfollowing treatment with CAP-34. The glucanase induction started within 3 h oftreatment, and maintained a peak value between 6 and 24 h before declining between48 and 72 h. Two isoforms were purified from resistant C. tetragonoloba. Both were basic and possessed an Mr of 34 and 36 kDa. Their pI was greater than pH 9.3. p Hoptima were 5.5 and 5.6 for the 34 and 36 kDa isoforms, respectively, while their Kmwas 400 and 666.6mg ml–1. While the total yield of the 36 kDa glucanase was considerably higher than the 34 kDa isoform, its specific activity was less. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Israel Journal of Plant Sciences Brill

Purification of Two Basic 1, 3-b-Glucanase Isoforms from Cyamopsis tetragonoloba (L.)Taub. Induced to Resist Virus Infections

Download PDF
5 pages

Loading next page...
 
/lp/brill/purification-of-two-basic-1-3-b-glucanase-isoforms-from-cyamopsis-kB9KB561Hf

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0792-9978
DOI
10.1560/7UWD-RGNP-CLFR-B4C5
Publisher site
See Article on Publisher Site

Abstract

1,3-b-glucanases (EC 3.2.1.39) are enzymes that degrade polysaccharidic substratesand have antimicrobial activity against fungi and bacteria. They have also been implicated in systemic acquired resistance (SAR) indirectly through release of endogenous elicitor molecules. SAR against viruses can be induced in plants by a few substances, including proteins isolated from some non-host plants. In the present study, CAP-34, a 34 kDa basic protein isolated from Clerodendrumaculeatum L., was used to induce systemic resistance against sunnhemp rosette virusin Cyamopsis tetragonoloba (L.) Taub. 1,3-b-glucanase activity increased rapidlyfollowing treatment with CAP-34. The glucanase induction started within 3 h oftreatment, and maintained a peak value between 6 and 24 h before declining between48 and 72 h. Two isoforms were purified from resistant C. tetragonoloba. Both were basic and possessed an Mr of 34 and 36 kDa. Their pI was greater than pH 9.3. p Hoptima were 5.5 and 5.6 for the 34 and 36 kDa isoforms, respectively, while their Kmwas 400 and 666.6mg ml–1. While the total yield of the 36 kDa glucanase was considerably higher than the 34 kDa isoform, its specific activity was less.

Journal

Israel Journal of Plant Sciences – Brill

Published: May 13, 2001

There are no references for this article.