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ACCUMULATION OF TRYPAN BLUE AND TRYPAN RED IN NEPHROCYTES OF THE FRESH-WATER SHRIMP NEOCARIDINA DENTICULATA (DECAPODA: ATYIDAE)

ACCUMULATION OF TRYPAN BLUE AND TRYPAN RED IN NEPHROCYTES OF THE FRESH-WATER SHRIMP NEOCARIDINA... and The small fresh-water shrimp Neocaridina denticulata (de Haan), an inhabitant of rivers, is known to migrate. To study its migration, marking with two kinds of vital stains, trypan blue and trypan red, was carried out by Costello (1964) and Niwa (1994). Both stains accumulated in the gills after injection, ap- pearing as marked chevron-shaped patterns beneath the branchiostegite. This marking does not subsequently fade. In spite of the strong toxicity of the pigment to crustaceans, the shrimp can survive 50 times the amount given to the crayfish Procambarus clarkii (Girard) (see Ueno, in preparation). The ex- istence of nephrocytes in the gill is well known from earlier investigations (Cuenot, 1895; Hewitt, 1907; Drach, 1930; Vogt, 1931; Balss, 1944; reviewed by Johnson, 1987), and the relationship between vital stains and nephrocytes was discussed by Drach (1930), who used trypan blue. Vogt (1931) used many different stains to identify the nephrocyte. In addition to trypan red, carmine and indigo- carmine were also used, but he obtained best results with trypan blue. The studies by Cuenot, Drach, and Vogt showed that nephro- cytes are found in decapods and mysids. All these observations were made by light mi- croscopy, however, and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Crustacean Biology Brill

ACCUMULATION OF TRYPAN BLUE AND TRYPAN RED IN NEPHROCYTES OF THE FRESH-WATER SHRIMP NEOCARIDINA DENTICULATA (DECAPODA: ATYIDAE)

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Publisher
Brill
Copyright
Copyright 1998 by Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0278-0372
eISSN
1937-240X
DOI
10.1163/193724098X00520
Publisher site
See Article on Publisher Site

Abstract

and The small fresh-water shrimp Neocaridina denticulata (de Haan), an inhabitant of rivers, is known to migrate. To study its migration, marking with two kinds of vital stains, trypan blue and trypan red, was carried out by Costello (1964) and Niwa (1994). Both stains accumulated in the gills after injection, ap- pearing as marked chevron-shaped patterns beneath the branchiostegite. This marking does not subsequently fade. In spite of the strong toxicity of the pigment to crustaceans, the shrimp can survive 50 times the amount given to the crayfish Procambarus clarkii (Girard) (see Ueno, in preparation). The ex- istence of nephrocytes in the gill is well known from earlier investigations (Cuenot, 1895; Hewitt, 1907; Drach, 1930; Vogt, 1931; Balss, 1944; reviewed by Johnson, 1987), and the relationship between vital stains and nephrocytes was discussed by Drach (1930), who used trypan blue. Vogt (1931) used many different stains to identify the nephrocyte. In addition to trypan red, carmine and indigo- carmine were also used, but he obtained best results with trypan blue. The studies by Cuenot, Drach, and Vogt showed that nephro- cytes are found in decapods and mysids. All these observations were made by light mi- croscopy, however, and

Journal

Journal of Crustacean BiologyBrill

Published: Jan 1, 1998

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