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Immunological Differentiation Between Bursaphelenchus Xylophilus and B. Mucronatus

Immunological Differentiation Between Bursaphelenchus Xylophilus and B. Mucronatus IMMUNOLOGICAL DIFFERENTIATION BETWEEN BURSAPHELENCHUS XYLOPHILUS AND B. MUCRONATUS BY C. LAWLER1) P. JOYCE2) and M. A. HARMEY1) 1) Dept. of Botany, University College Dublin, Dublin 4, Ireland; 2) Zoology Dept., University College Dublin Antisera to Bursaphelenchus xylophilus was prepared by two different immunisation protocols. The antibodies were examined using the Enzyme Linked Immunoabsorbent Assay (ELISA) and West- ern Blots. The polyclonal antibodies obtained by the different immunisation schedules did not clearly distinguish B. xylophilus from B. mucronatus using the ELISA system. The use of rats immunotolerised to B. mucronatus antigen produced polyclonal antibodies which could distinguish specific antigens of certain B. xylofihilus populations when examined using Western Blots. The banding patterns of the B. xylophilus isolates examined using the polyclonal antibodies obtained from immunotolerised rats indicate that Japanese and United States populations are more closely related to each other than to the Canadian populations. Keywords: Bursaphelenchus xylophilus, Bursaphelenchus mucronatus, Bursaphelenchus fraudulentus, ELISA, Western Blots, Immunotolerised The unequivocal identification and differentiation of Bursaphelenenchus xylo- philus and B. mucronatus has posed a problem for nematologists ever since the 1970s when the two nematodes were first identified (Mamiya & Kiyohara, 1972; Mamiya & Enda, 1979). The identification of the two nematode http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Immunological Differentiation Between Bursaphelenchus Xylophilus and B. Mucronatus

Lawler, C.; Joyce, P.; Harmey, M.A.
Nematologica , Volume 39 (1-4): 11 – Jan 1, 1993
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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529293X00475
Publisher site
See Article on Publisher Site

Abstract

IMMUNOLOGICAL DIFFERENTIATION BETWEEN BURSAPHELENCHUS XYLOPHILUS AND B. MUCRONATUS BY C. LAWLER1) P. JOYCE2) and M. A. HARMEY1) 1) Dept. of Botany, University College Dublin, Dublin 4, Ireland; 2) Zoology Dept., University College Dublin Antisera to Bursaphelenchus xylophilus was prepared by two different immunisation protocols. The antibodies were examined using the Enzyme Linked Immunoabsorbent Assay (ELISA) and West- ern Blots. The polyclonal antibodies obtained by the different immunisation schedules did not clearly distinguish B. xylophilus from B. mucronatus using the ELISA system. The use of rats immunotolerised to B. mucronatus antigen produced polyclonal antibodies which could distinguish specific antigens of certain B. xylofihilus populations when examined using Western Blots. The banding patterns of the B. xylophilus isolates examined using the polyclonal antibodies obtained from immunotolerised rats indicate that Japanese and United States populations are more closely related to each other than to the Canadian populations. Keywords: Bursaphelenchus xylophilus, Bursaphelenchus mucronatus, Bursaphelenchus fraudulentus, ELISA, Western Blots, Immunotolerised The unequivocal identification and differentiation of Bursaphelenenchus xylo- philus and B. mucronatus has posed a problem for nematologists ever since the 1970s when the two nematodes were first identified (Mamiya & Kiyohara, 1972; Mamiya & Enda, 1979). The identification of the two nematode

Journal

Nematologica – Brill

Published: Jan 1, 1993

Keywords: ELISA; Bursaphelenchus fraudulentus; Western Blots; Bursaphelenchus mucronatus; Immunotolerised; Bursaphelenchus xylophilus

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