Access the full text.
Sign up today, get DeepDyve free for 14 days.
References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.
SHORT COMMUNICATIONS L. V. LOPEZ-LLORCA & J. M. DUNCAN1): New media for the estimation of fungal infection in eggs of the cereal cyst nematode, Heterodera avenae Woll. Various soil fungi, mainly hyphomycetes, parasitize eggs of the cereal cyst nematode Heterodera avenae Woll., and of other cyst nematodes (Tribe, 1977; Kerry, 1981; Morgan-Jones & Rodriguez-Kabana, 1981). Assessing the extent of parasitism usually involves plating eggs on a nutrient-poor medium and counting the numbers from which fungi develop (Kerry & Crump, 1977; Nigh et al., 1980; Dackman & Nordbring-Hertz, 1985). The method has limitations because hyphal growth is generally too sparse to allow identification without sub-culturing but rapid enough to cause overlap between colonies resulting in inaccurate counts. In this note we describe two media which, when used together, can overcome these difficulties. Medium 1 was 1 % distilled water agar and Medium 2 was 1 % peptone dex- trose agar to both of which were added 1000 ¡.tg ml-I Triton-X 100, 50 Ag ml-I streptomycin sulphate, 50 /tg ml-I aureomycin (chlortetracyline hydrochloride), and 50 /tg ml-1 rose bengal. The agars were poured into 90 mm diam. plastic dishes, to the undersides of which were attached clear acetate grids, 32
Nematologica – Brill
Published: Jan 1, 1986
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.