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New media for the estimation of fungal infection in eggs of the cereal cyst nematode, Heterodera avenae Woll

New media for the estimation of fungal infection in eggs of the cereal cyst nematode, Heterodera... SHORT COMMUNICATIONS L. V. LOPEZ-LLORCA & J. M. DUNCAN1): New media for the estimation of fungal infection in eggs of the cereal cyst nematode, Heterodera avenae Woll. Various soil fungi, mainly hyphomycetes, parasitize eggs of the cereal cyst nematode Heterodera avenae Woll., and of other cyst nematodes (Tribe, 1977; Kerry, 1981; Morgan-Jones & Rodriguez-Kabana, 1981). Assessing the extent of parasitism usually involves plating eggs on a nutrient-poor medium and counting the numbers from which fungi develop (Kerry & Crump, 1977; Nigh et al., 1980; Dackman & Nordbring-Hertz, 1985). The method has limitations because hyphal growth is generally too sparse to allow identification without sub-culturing but rapid enough to cause overlap between colonies resulting in inaccurate counts. In this note we describe two media which, when used together, can overcome these difficulties. Medium 1 was 1 % distilled water agar and Medium 2 was 1 % peptone dex- trose agar to both of which were added 1000 ¡.tg ml-I Triton-X 100, 50 Ag ml-I streptomycin sulphate, 50 /tg ml-I aureomycin (chlortetracyline hydrochloride), and 50 /tg ml-1 rose bengal. The agars were poured into 90 mm diam. plastic dishes, to the undersides of which were attached clear acetate grids, 32 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

New media for the estimation of fungal infection in eggs of the cereal cyst nematode, Heterodera avenae Woll

Nematologica , Volume 32 (4): 4 – Jan 1, 1986

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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529286X00354
Publisher site
See Article on Publisher Site

Abstract

SHORT COMMUNICATIONS L. V. LOPEZ-LLORCA & J. M. DUNCAN1): New media for the estimation of fungal infection in eggs of the cereal cyst nematode, Heterodera avenae Woll. Various soil fungi, mainly hyphomycetes, parasitize eggs of the cereal cyst nematode Heterodera avenae Woll., and of other cyst nematodes (Tribe, 1977; Kerry, 1981; Morgan-Jones & Rodriguez-Kabana, 1981). Assessing the extent of parasitism usually involves plating eggs on a nutrient-poor medium and counting the numbers from which fungi develop (Kerry & Crump, 1977; Nigh et al., 1980; Dackman & Nordbring-Hertz, 1985). The method has limitations because hyphal growth is generally too sparse to allow identification without sub-culturing but rapid enough to cause overlap between colonies resulting in inaccurate counts. In this note we describe two media which, when used together, can overcome these difficulties. Medium 1 was 1 % distilled water agar and Medium 2 was 1 % peptone dex- trose agar to both of which were added 1000 ¡.tg ml-I Triton-X 100, 50 Ag ml-I streptomycin sulphate, 50 /tg ml-I aureomycin (chlortetracyline hydrochloride), and 50 /tg ml-1 rose bengal. The agars were poured into 90 mm diam. plastic dishes, to the undersides of which were attached clear acetate grids, 32

Journal

NematologicaBrill

Published: Jan 1, 1986

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