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A modified apparatus for the extraction o f nematode.r f rom sterile culture

A modified apparatus for the extraction o f nematode.r f rom sterile culture SHORT COMMUNICATIONS R. M. WEBB1) : A modified apparatus for the extraction o f nematode.r f rom sterile culture. Webb (1971) described an apparatus for extracting nematodes from infested, monoxenic cultures aseptically. However, the stopcocks frequently split during autoclaving, even when fitted with PTFE taps. To overcome this problem the apparatus was modified as in Fig. 1. 1) Rothamsted Experimental Station, Harpenden, England. 409 Funnel A, cotton wool base filling B and vent tube E are as described previously. Tap C in the former apparatus is replaced with an S bend of 2 mm internal diam. capillary tubing F, passing through a rubber bung instead of a cork, and tube D is changed to a 100 ml conical flask to give stability. Distilled water is added to funnel A until the cotton wool B is just covered. Funnel A is then capped with aluminium foil secured with wire stem and the apparatus autoclaved at 15 p.s.i. for 15 min. Nematode infested aseptic plant tissue is placed in funnel A under sterile con- ditions. The funnel is recapped and the apparatus left to stand at room temperature for 48 hr to allow worms to pass out of the tissue http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

A modified apparatus for the extraction o f nematode.r f rom sterile culture

Nematologica , Volume 21 (3): 2 – Jan 1, 1975

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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529275X00149
Publisher site
See Article on Publisher Site

Abstract

SHORT COMMUNICATIONS R. M. WEBB1) : A modified apparatus for the extraction o f nematode.r f rom sterile culture. Webb (1971) described an apparatus for extracting nematodes from infested, monoxenic cultures aseptically. However, the stopcocks frequently split during autoclaving, even when fitted with PTFE taps. To overcome this problem the apparatus was modified as in Fig. 1. 1) Rothamsted Experimental Station, Harpenden, England. 409 Funnel A, cotton wool base filling B and vent tube E are as described previously. Tap C in the former apparatus is replaced with an S bend of 2 mm internal diam. capillary tubing F, passing through a rubber bung instead of a cork, and tube D is changed to a 100 ml conical flask to give stability. Distilled water is added to funnel A until the cotton wool B is just covered. Funnel A is then capped with aluminium foil secured with wire stem and the apparatus autoclaved at 15 p.s.i. for 15 min. Nematode infested aseptic plant tissue is placed in funnel A under sterile con- ditions. The funnel is recapped and the apparatus left to stand at room temperature for 48 hr to allow worms to pass out of the tissue

Journal

NematologicaBrill

Published: Jan 1, 1975

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