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Sealing microscopical water mounts with soft wax

Sealing microscopical water mounts with soft wax 258 Table I compares cysts of H. tabacum from Connecticut with cysts of H. rortochien.ri.r from 25 British populations and includes Granek's measurements. The ratios from H. tabacum cysts agree with those of Granek but the range is greater, the mean slightly higher and the percentages of cysts with values below 1.5, 2.0 and 3.0 are less. The values from British populations of H. ro.rto- chien.ri.r are lower than for the Long Island population and the range overlaps that of H. tabacum. Although Granek's ratio would rarely fail to distinguish between Connecticut tobacco cysts and Long Island potato cysts, it would often fail to differentiate between tobacco cysts and potato cysts from Britain. Measurements of a few potato cysts from India gave ratios within the British range. There were no differences between different types of British population. GRANEK, I. (1955). Additional morphological differences between cysts of Heterodera rostochiensis and Heterodera tabacum. Pl. Dis. Reptr, 39, 716-718. SHORT COMMUNICATIONS C. C. DONCASTER 1) : Sealing microscopical water mounts with soft wax. When mounting specimens in water on microscope slides of temporary use, an ordinary candle, recently extinguished can be used as a brush to apply a ring of wax round the edge of the coverslip (Goodey, 1957). Even after applying a thick wax seal, water slowly evaporates from beneath the coverglass because, on cooling, the wax cr= cks immediately over the sharp edge of the coverslip. More lasting water mounts can be made using a pliable wax made by mixing paraffin embedding wax or candle wax with a little Vaseline (petroleum jelly). 1 to 2 volumes of Vaseline mixed with 4 volumes of paraffin is satisfactory. The moulten mixture is poured into a glass tube about 15 cm long by about 0.8 cm internal diameter. The bottom of the tube is first sealed with a cork through the middle of which is threaded a length of cotton dish cloth yarn as a wick. The lower end of the yarn is held tightly by the cork and the upper end is held taut, coaxially with the tube, while the wax-Vaseline mixture sets around it. On removing the cork and trimming the wick, the candle can be pushed a few millimetres out of one end of the tube with a pencil or glass rod. The wick is lit, allowed to saturate with moulten wax and then extinguished and used as a brush for applying the cover glass seal. GOODEY, J. B., 1957. Laboratory Methods for Work with Plant and Soil Nematodes. Techn. Bull. 2, Minist. Agric. London, H.M.S.O. 3rd edition. 47 pp. 1) Rothamsted Experimental Station, Harpenden, Herts., England. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Sealing microscopical water mounts with soft wax

Nematologica , Volume 7 (3): 1 – Jan 1, 1962

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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
DOI
10.1163/187529262X00279
Publisher site
See Article on Publisher Site

Abstract

258 Table I compares cysts of H. tabacum from Connecticut with cysts of H. rortochien.ri.r from 25 British populations and includes Granek's measurements. The ratios from H. tabacum cysts agree with those of Granek but the range is greater, the mean slightly higher and the percentages of cysts with values below 1.5, 2.0 and 3.0 are less. The values from British populations of H. ro.rto- chien.ri.r are lower than for the Long Island population and the range overlaps that of H. tabacum. Although Granek's ratio would rarely fail to distinguish between Connecticut tobacco cysts and Long Island potato cysts, it would often fail to differentiate between tobacco cysts and potato cysts from Britain. Measurements of a few potato cysts from India gave ratios within the British range. There were no differences between different types of British population. GRANEK, I. (1955). Additional morphological differences between cysts of Heterodera rostochiensis and Heterodera tabacum. Pl. Dis. Reptr, 39, 716-718. SHORT COMMUNICATIONS C. C. DONCASTER 1) : Sealing microscopical water mounts with soft wax. When mounting specimens in water on microscope slides of temporary use, an ordinary candle, recently extinguished can be used as a brush to apply a ring of wax round the edge of the coverslip (Goodey, 1957). Even after applying a thick wax seal, water slowly evaporates from beneath the coverglass because, on cooling, the wax cr= cks immediately over the sharp edge of the coverslip. More lasting water mounts can be made using a pliable wax made by mixing paraffin embedding wax or candle wax with a little Vaseline (petroleum jelly). 1 to 2 volumes of Vaseline mixed with 4 volumes of paraffin is satisfactory. The moulten mixture is poured into a glass tube about 15 cm long by about 0.8 cm internal diameter. The bottom of the tube is first sealed with a cork through the middle of which is threaded a length of cotton dish cloth yarn as a wick. The lower end of the yarn is held tightly by the cork and the upper end is held taut, coaxially with the tube, while the wax-Vaseline mixture sets around it. On removing the cork and trimming the wick, the candle can be pushed a few millimetres out of one end of the tube with a pencil or glass rod. The wick is lit, allowed to saturate with moulten wax and then extinguished and used as a brush for applying the cover glass seal. GOODEY, J. B., 1957. Laboratory Methods for Work with Plant and Soil Nematodes. Techn. Bull. 2, Minist. Agric. London, H.M.S.O. 3rd edition. 47 pp. 1) Rothamsted Experimental Station, Harpenden, Herts., England.

Journal

NematologicaBrill

Published: Jan 1, 1962

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